云南农业大学学报2012，Vol.27Issue(4)：579-584,6.DOI:10.3969/j.issn.1004-390X(n).2012.04.021
青花菜单倍体育种研究进展
Advances in Haploid Breeding of Broccoli ( Brassica oleracea var.italica)
摘要
Abstract
The use of microspore or anther culture to generate doubled-haploids ( DH ) is an important adjunct to broccoli breeding. In this paper, the history and the recent progress on anther culture and i-solated microspore culture of broccoli was reviewed. Anther culture technique, isolated microspore culture technique and the major factors affecting the formation of plant regeneration were expounded. Ploidy compositions of populations derived from microspore culture and from anther culture were compared. The main determination methods of ploidy were introduced in detail. The application status and the theory value of haploidy and DH lines were summarized as well. The problems of haploidy breeding of broccoli were discussed, and the application of the techniques in study and practice was forecasted.关键词
青花菜/单倍体育种/花药培养/小孢子培养/DH系Key words
broccoli/ haploid breeding/ anther culture/ microspore culture/ DH lines分类
园艺学与植物营养学引用本文复制引用
兰梅,黄丽,许彬,张应华..青花菜单倍体育种研究进展[J].云南农业大学学报,2012,27(4):579-584,6.基金项目
云南省教育厅科学研究基金项目 (09C0264). （09C0264）
