医药导报2012，Vol.31Issue(5)：581-583,3.DOI:10.3870/yydb.2012.05.008
七氟烷对兔心肌缺血-再灌注心律失常及NADPH氧化酶亚单位p22phox表达的影响
Effects of Sevoflurane on Arrhythmia Induced by Myocardial Ischemia/reperfusion in Rabbits and Expression of NADPH Oxidase Subunit p22phox
张俊峰 1周武 1王涛2
作者信息
- 1. 湖北省新华医院麻醉科,武汉430015
- 2. 华中科技大学同济医学院药学院,武汉430030
- 折叠
摘要
Abstract
Objective To explore Ihe effects of sevoflurane on arrhythmia induced by myocardial ischemia/reperfusion in rabbits and expression of NADPH oxidase subunit p22phon. Methods The model of myoeardial ischemia/reperfusion was established in rabbits,and which were randomly divided into 3 groups: sham-operated group,ischemia-reperfusion(I/R) group and sevoflurane-pretreatment group. The level of lipid peroxidate ( LPO) was detected by spectrophotometry with thiobarbituric acid reaction products(TBARS). The expression of NADPH oxidase subunit p22phon was assayed by western blotting. Results The incidence of ventricular arrhythmias in the model group was 90% .with duration as [ (158.82±18. 86)s] ,both of which were markbly up-regulated in comparison with the sham-operated group. The level of myocardial LPO in the model group was [ (2. 12± 0. 36) μg o g-1 ],which was significantly higher than that of the sham-operated group [ (0. 89±0. 11) μg·g-1 ,P<0. 01 ]. The expression of p22phon subunit was obviously up-regulated. Compared with the model control. the incidence of ventricular arrhythmias (70% ), duration [(39. 65 ±5. 27) s] , myoranlial LPO [(1.35±0.20)μg·g-1 ] and p22phon expression in the sevoflurane-pretreatmenl group were significant down-regulated. Conclusion Sevoflurane could suppress arrhythmia induced by myocardial I/R in rabbits,the mechanism of which involved with down-regulating p22phon expression and therefore dropping the level of LPO in tissues.关键词
七氟烷/缺血-再灌注/心律失常/NADPH氧化酶Key words
Sevoflurane/ Ischemia/reperfusion/ Arrhythmia/ NADPH oxidase分类
医药卫生引用本文复制引用
张俊峰,周武,王涛..七氟烷对兔心肌缺血-再灌注心律失常及NADPH氧化酶亚单位p22phox表达的影响[J].医药导报,2012,31(5):581-583,3.
