医药导报2012，Vol.31Issue(5)：584-588,5.DOI:10.3870/yydb.2012.05.009
高效液相色谱串联质谱法测定人血浆硝苯地平浓度
Determination of Nifedipine in Human Plasma by High Performance Liquid Chromatography Tandem Mass Spectrometry
任秀华 1袁春平 2张冬林 1刘宇 1陈倩 1刘东1
作者信息
- 1. 华中科技大学同济医学院附属同济医院药学部,武汉430030
- 2. 广东环球制药有限公司,广东佛山528303
- 折叠
摘要
Abstract
Objective To establish a high performance liquid chromatography tandem mass spectrometry( HPLC-MS/ MS) method for determinng nifedipine in human plasma. Methods Plasma samples were precipitated with acetonitrile and detected by HPLC-MS/MS using tnidazolam as internal standard. The separation was carried out on a Diamonsil Cl8 column (2. 1 mm×l50 mm, 5μm)with a mobile phase of methanol-H2O(77. 7 : 23. 3) and the flow rate was 0. 3 mL·min-1. Electrospray ionization( ESI) was performed,and the mulli-reaction monitoring mode(MRM) was used to monitor positive ion and the quantitative analysis of ion pairs for nifedipine and internal standard midazolam was at m/z 369. 1→224. 2 and m/z 325.9→ 291.0,respectively. Results The linear range of nifedipine in plasma was within 1.205-241.000 ng·mL-1 ,r = 0.996 9. The LLOQ of nifedipine in plasma was 1.205 ng· mL-1. The relative recovery of the method was 85% -115% and the intra- and inter-day RSDs were less than 9%. Conclusion This method is sensitive, accurate, reliable and is suitable for pharmacokinetic studies of nifedipine in human.关键词
硝苯地平/血浆浓度/药动学/色谱法,高效液相/质谱法Key words
Nifedipine/ Plasma concentration/ Pharmacokinetics/ HPLC/ Mass spectrometry分类
医药卫生引用本文复制引用
任秀华,袁春平,张冬林,刘宇,陈倩,刘东..高效液相色谱串联质谱法测定人血浆硝苯地平浓度[J].医药导报,2012,31(5):584-588,5.
