医药导报2012，Vol.31Issue(5)：591-594,4.DOI:10.3870/yydb.2012.05.011
荧光法测定溶剂对抗生素与人血清清蛋白结合作用的影响
Determine the Influence of Solvents on Conjugation between Antibiotics with Human Serum Albumins by Fluorescence Spectrometry
林迦勒 1戴歌心 1张振南 2王学宝2
作者信息
- 1. 温州医学院附属第一医院药剂科,325000
- 2. 温州医学院药学院,325000
- 折叠
摘要
Abstract
Objective To investigate the influence of different solvents on conjugation of ceftazidime, ceftriaxone, cefminox and moxulactam with human serum albumins ( HSA) under physiological condition. Methods The apparent quenching constant(Kq) and binding constant(KA) between drugs and HSA were calculated by detecting the fluorescence values with fluorescence spectrometry. Results The interaction of ceftazidime,ceftriaxone,cefminox and moxalactam with HSA was a kind of static quenching procedure. The binding capacity with albumin of ceftazidime, ceftriaxone and cefminox in 5% GS was lower than that in 0.9% NS,while that of moxalactam increased to some extent. Conclusion The drug efficacy of ceftazidime, ceftriaxone and cefminox is more durable when taking 0.9% NS as solvent than 5% GS; while the drug effiacy of moxalactam is more durable when taking 5% GS as solvent than 0.9% NS.关键词
头孢他啶/头孢曲松/头孢米诺/拉氧头孢/光谱法,荧光Key words
Ceftazidime/ Ceftriaxone/ Cefminox/ Moxalactam/ Fluorescence spectrometry分类
医药卫生引用本文复制引用
林迦勒,戴歌心,张振南,王学宝..荧光法测定溶剂对抗生素与人血清清蛋白结合作用的影响[J].医药导报,2012,31(5):591-594,4.
