应用化学2012，Vol.29Issue(5)：591-596,6.DOI:10.3724/Sp.J.1095.2012.00269
老鼠血清中苯扎贝特的HPLC-MS法测定
Quantitative Determination of Bezafibrate in Rat Plasma Using HPLC-MS
曹慧慧 1彭敬东 1张蕾1
作者信息
- 1. 西南大学化学化工学院,发光与实时分析教育部重点实验室 重庆 400715
- 折叠
摘要
Abstract
A simple, rapid and sensitive HPLC-MS method was developed and validated for determining bezafibrate in rat plasma. Chromatographic separation was achieved on a reversed-phase Gemini C18 at 35 ℃ using acetonitrile, methanol and 0. 03% aqueous solution of formic acid( volume ratio 60:15: 25) as mobile phase at flow rate of 0. 3 mL/min. The UV absorption wavelength was set at 235 run and the injection volume was 5 μL. Under these conditions, the detection was performed with negative electrospray ionization, monitoring the transitions m/z 359. 65-273. 70 for bezafibrate and m/z 212. 95-127. 08 for the clofibric acid (internal standard). A good linearity was achieved within the concentration range of 0. 073 -7.884 mg/L (r =0. 9995) for bezafibrate and the mean recovery of samples ranged from 94. 3% to 103. 1% at three tested concentrations. The intra-day and inter-day precisions(as relative standard deviation) were below 6%. The limit of quantification (LOQ) was 30 μg/L and the limit of detection ( LOD) was 9μg/L. This HPLC-MS method was reproducible and sensitive enough for routine analysis of bezafibrate in human plasma.关键词
苯扎贝特/HPLC-MS/老鼠血清Key words
bezafibrate,HPLC-MS,rat plasma分类
化学化工引用本文复制引用
曹慧慧,彭敬东,张蕾..老鼠血清中苯扎贝特的HPLC-MS法测定[J].应用化学,2012,29(5):591-596,6.
