应用化学2012,Vol.29Issue(6):697-704,8.DOI:10.3724/SP.J.1095.2012.00314
反萃分散组合液膜分离提取氨基酸
Separation and Extraction of Amino Acids with Strip Dispersion Hybrid Liquid Membrane
摘要
Abstract
A method on the separation and extraction of Z,-methionine( Met), L-leucine( Leu) , L-phenylalanine (Phe) , and L-tryptophan(Trp) , using a strip dispersion hybrid liquid membrane(SDHLM) containing di-(2-ethylhexyl) phosphoric acid( D2EHPA) with kerosene as the organic liquid membrane phase and HC1 solution as the aqueous strip solution, is developed. The effects of pH in the feed solution, the concentration of the carrier( D2EHPA) , the volume ratio of the organic liquid membrane solution to the aqueous strip solution, the strip solution composition, the flow rates of the feed solution and the strip dispersion phase, the extraction time, and reuse times of the support membrane on the permeability coefficient and the transport efficiency of amino acids were investigated. Under the optimal conditions, the transport efficiencies obtained for the four amino acids, Met, Leu, Phe, and Trp were all higher than 35% , and more than 79% of transport efficiencies were obtained for Leu and Trp. The transport efficiency exhibits a trend of Et,Trp >Et,Leu >Et,Phe >Et,Met. With the 12 h-extraction time, the support membrane could be reused for at least 25 times with little decrease in the transport efficiency of amino acids. The developed method with high transport efficiency and excellent transport selectivity for the studied amino acids is simple and environmental friendly.关键词
反萃分散组合液膜/氨基酸/蛋氨酸/亮氨酸/苯丙氨酸/色氨酸/传输Key words
strip dispersion hybrid liquid membrane, amino acids, L-methionine, L-leucine, L-phenylalanine, L-tryptophan, transport分类
化学化工引用本文复制引用
陈园园,商静芬,欧阳丽,苏孝礼,方正法,马铭..反萃分散组合液膜分离提取氨基酸[J].应用化学,2012,29(6):697-704,8.基金项目
国家自然科学基金(20875029,20975038)资助项目 (20875029,20975038)