针刺研究2012，Vol.37Issue(3)：191-196,6.
电针“扶突”等穴对颈部切口痛大鼠痛行为及脊髓mGluR5/cAMP/CREB信号通路活动的影响
Effects of Electroacupuncture at “Futu”(LI 18),etc.on Pain Threshold and Cervico-spinal mGlu Receptor 5/cAMP/CREB Signaling in Rats with Neck Incision Pain
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) at "Futu" (LI 18),etc. on the pain threshold and expression of metabotropic glutamate receptor subtype 5 (mGluR 5), cyclic adenosine monophosphate (cAMP), mitogen-ac-tivated protein kinase (MAPK), cAMP responsive element binding protein (CREB) genes in the cervico-spinal dorsal region in rats with neck incision pain so as to analyze its underlying analgesic mechanism in performing thyroid surgery. Methods Forty male Wistar rats were randomly divided into control, model, Futu (L118), Hegu (LI 4)-Neiguan (PC 6), Zusanli (ST 36)-Yangiingquan (GB 34) groups, with 8 cases in each group. Neck-incision pain model was established by making a longitudinal incision along the midline of the neck under anesthesia (isoflurane) except rats in the control group. EA(2 Hz/15 Hz, 1-2 mA) was applied to bilateral LI 18, LI 4-PC 6 and ST 36-GB 34 for 30 min, 4 h, 24 h and 48 h after modeling, respectively. The thermal pain threshold was detected before modeling, and before and after each treatment. After removing the ventral part, the spinal tissue of the cervical) - 4 segments was collected for detecting the expression of mGluR 5, cAMP, MAPK, CREB genes by using quantitative real-time-PCR technique. Results In comparison with the control group, the pain threshold of the model group was decreased obviously (P<0.05) . Compared with the model group, the pain thresholds of the LI 18 and LI 4-PC 6 groups were upregulated markedly after treatment (P<0. 05). No significant differences were found between the LI 18 and LI 4-PC 6 groups, and between the model and ST 36-GB 34 groups in pain thresholds (P>0. 05). Compared with the control group, the expression le-vels of mGluR 5 mRNA, cAMP mRNA and CREB mRNA in the cervico-spinal cord were upregulated significantly in the model group (P<0. 05). While in comparison with the model group, cervico-spinal cAMP mRNA, and CREB mRNA were down-regulated markedly (P<0. 05) and mGluR 5 mRNA was down-regulated moderately(P>0.05) in the LI 18 group, rather than in the LI 4-PC 6 and ST 36-GB 34 groups (P>0. 05). Conclusion EA-LI 18 can significantly suppress neck incision pain induced pain reaction, which may be closely associated with its functions in down-regulating expression levels of intracellular cAMP mRNA and CREB mRNA in the cervico-spinal cord.关键词
颈部切口痛/电针镇痛/脊髓/mGluR 5/cAMP/CREB/MAPK/基因表达/信号通路Key words
Cervical incision pain/ Acupuncture analgesia/ Spinal cord/ Gene expression/ Metabotropic glutamate receptor subtype 5/ cAMP/MAPK/CREB signaling分类
医药卫生引用本文复制引用
林丹,阚宇,乔丽娜,王俊荚,陈淑萍,韩焱晶,张建梁,刘俊岭..电针“扶突”等穴对颈部切口痛大鼠痛行为及脊髓mGluR5/cAMP/CREB信号通路活动的影响[J].针刺研究,2012,37(3):191-196,6.基金项目
项目来源:国家“973”计划中医理论研究专项(No.2007CB512505)、国家自然科学基金项目(No.30973796、No.30973796)、国家自然科学基金重大项目(No.90709031) （No.2007CB512505）
