针刺研究2012，Vol.37Issue(3)：211-217,7.
电针大鼠耳穴“心”等对孤束核升压反射相关神经元电活动的影响
Effect of Electroacupuncture on Electrical Activities of Pressor Reflex Related Neurons in the Nucleus of Tractus Solitarii(NTS)in Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)of auricular point "Xin" (Heart ),"Neiguan" (PC 6)and "Zusanli" (ST 36) on electrical activities of the pressor reflex-related neurons in the nucleus of the solitary tract(NTS) in rats. Methods A total of 47 SD rats were used in the present study. Extracellular electrical activities of neurons in the NTS (AP:1. 0 mm. Left and right:0- 1. 0 mm, depth:0. 3-0. 8 mm)were recorded by using glass mlcroelectrodes(10- 15 MΩ). Carotid arterial pressure changes were monitored and recorded by using a pressure transducer. EA(2 mA. 10 Hz, duration; 500 ms) was randomly applied to the left auricular point "Xin", "Neiguan" (PC 6)and "Zusanli" (ST36) for 30 s, respectively. Elevation of arterial pressure was induced by intravenous in/ection of Phenylephrine (PE, 6 - 12 ug/kg, 2 μg/mL). Results Of the 67 NTS neurons, 15 were activated by intravenous injection of PE (which were called as pressor reflex-related neurons). Following EA of the auricular point "Xin" and ST36. the mean arterial pressure (MAP) levels in normal rats and PE-induced MAP increase rats were lowered significantly < P<0. 001, P<0. 05). Concomitantly, the firing rates of pressor reflex-related NTS neurons were increased considerably by EA stimulation of auricular point "Xin" and ST 36, but not by EA of PC 6. EA of PC 6 had no apparent influence on MAP in normal rats and in PE-induced MAP increase rats (P>0.05). Conclusion Electroacupuncture stimulation of auricular point "Xin" and "Zusanli" (ST36) can lower MAP in normal rats and PE-induced MAP elevation rats, and enhance pressor reflex induced increase of firing rates of NTS neurons, suggesting an interaction between signals from electroacupuncture stimulation of "Xin" and "Zusanli" (ST 36) and from cardiovascular activities in the NTS neurons.关键词
电针/细胞外放电/孤束核/平均动脉压/升压反射相关神经元/耳穴“心”/“足三里”Key words
Electroacupuncture/ Extracellular discharge/ Nucleus of the solitary tract/ Mean arterial pressure/ Pressor reflex-related neurons/ Auricular point "Xin"/ Zusanli(ST 36)分类
医药卫生引用本文复制引用
刘坤,李亮,贲卉,赵玉雪,荣培晶,高昕妍,朱兵..电针大鼠耳穴“心”等对孤束核升压反射相关神经元电活动的影响[J].针刺研究,2012,37(3):211-217,7.基金项目
项目来源:国家自然科学基金项目(No.30371804)及“973”国家重点基础研究发展计划(No.2010CB505200) （No.30371804）
