诊断学理论与实践2012，Vol.11Issue(2)：130-135,6.DOI:10.3969/j.issn.1671-2870.2012.02.010
利妥昔单抗和RAD001对弥漫大B细胞株SUDHL-4和DB细胞增殖和凋亡的影响
Effect of RAD001 plus rituximab on proliferation and apoptosis of DLBCL cell line SUDHL-4 and DB cells
王文方 1徐子真 2王爱华 1诸江 3李军民1
作者信息
- 1. 上海交通大学医学院附属瑞金医院血液科
- 2. 上海交通大学医学院附属瑞金医院检验系
- 3. 上海血液学研究所,上海200025
- 折叠
摘要
Abstract
Objective To study the effects of rituximab,RAD001 and rituximab plus RAD001 on proliferation and apoptosis of diffuse large B-cell lymphoma cell line SUDHL-4 and DB cells. Methods MIT assay was used to assess cell proliferation. Flow cytometry was performed to examine cell cycle and apoptosis. Protein expression of mTOR signaling pathway,cell cycle and apoptosis related proteins were detected by Western blot. Result Rituximab accelerated apoptosis of SUDHL-4 and DB cells. RAD001 inhibited the proliferation of SUDHL-4 and DB cells and kept the cell in G/G1 phase,but did not have obvious effect on apoptosis. Western blot showed that rituximab plus RAD001 could decrease the activity of downstream molecules,down-regulate cell cycle related protein,and anti-apoptotic protein up-regulate apoptotic associated protein. Conclusions Rituximab and RAD001 could function cooperatively to inhibit the proliferation and promote the apoptosis of SUDHL-4 and DB cells.关键词
利妥昔单抗/RAD001/mTOR/弥漫大B细胞淋巴瘤/凋亡/细胞周期/SUDHL-4细胞/DB细胞Key words
Rituximab/RAD001/mTOR/Diffuse large B-cell lymphoma/Apoptosis/Cell cycle/SUDHL-4 cells/DB cells分类
医药卫生引用本文复制引用
王文方,徐子真,王爱华,诸江,李军民..利妥昔单抗和RAD001对弥漫大B细胞株SUDHL-4和DB细胞增殖和凋亡的影响[J].诊断学理论与实践,2012,11(2):130-135,6.
