诊断学理论与实践2012，Vol.11Issue(2)：141-144,4.DOI:10.3969/j.issn.1671-2870.2012.02.012
流式细胞术检测微小残留病预测儿童急性B淋巴细胞白血病复发的意义
Detection of minimal residual disease by flow cytometry as a useful approach for predicting relapse in childhood B-lingeage acute lymphoblastic leukemia
张芳 1汤燕静 1陈静 1李本尚 1王翔 1马亚妮 1薛惠良 1潘慈 1叶启东 1江华 1周敏1
作者信息
- 1. 上海交通大学医学院附属上海儿童医学中心血液肿瘤科,上海200127
- 折叠
摘要
Abstract
Objective To study the value of detection of minima) residual disease( MRD ) by flow cytometry (FCM) during the process of chemotherapy for predicting relapse in childhood B-lingeage acute lymphoblastic leukemia. Methods MRD was detected by four-color flow cytometry on day 19,day 35,day 55 (only for medium- or high-risk group) and week 13-17,week 28-36,week 52-54,week 78-83,week 102-107,last day of the chemotherapy process and during follow-up in 291 childhood B-lingeage acute lymphonlasric leukemia patients. Results (1)D MRD were <0.01%,0.01%-0.1%,0.1%-l%and >I% in 169,35,44,43 patients,respectively. The 7 years cumulative incidence of relapse were 4.1%,17.1%,29.5% and 62.8% in patients with MRD<0.01%,0.0l%-0.1%,0.1%-l%and >1%,respectively. The differences between these four groups were statistically significant (P<0.0001). (2) The difference in relapse rate was statistically significant between MRD positive and MRD negative patients at every detection time point. (P=0.0021 on day 19,P=0.0008 on day 55,P=0.0025 during follow-up,and other time points P<0.0001) (3)Day 35 was an important time point to predict early and late relapse,week 14-17 was an important time point to predict early relapse,and MRD positive during follow-up was valuable for predicting late relapse. Conclusions MRD detection by flow cytometry is valuable for predicting relapse of childhood B-ALL and has different significance at different time points.关键词
急性B淋巴细胞白血病/微小残留病/流式细胞术/复发/儿童Key words
B-iineage acute lymphoblastic leukemia/Minimal residual disease/Flow cytomety/Relapse/Children分类
医药卫生引用本文复制引用
张芳,汤燕静,陈静,李本尚,王翔,马亚妮,薛惠良,潘慈,叶启东,江华,周敏..流式细胞术检测微小残留病预测儿童急性B淋巴细胞白血病复发的意义[J].诊断学理论与实践,2012,11(2):141-144,4.
