中国病理生理杂志2012，Vol.28Issue(8)：1362-1366,5.DOI:10.3969/j.issn.1000-4718.2012.08.004
siRNA特异性沉默septin2基因抑制大肠癌LoVo细胞迁移
Inhibitory effects of septin 2 gene silencing induced by siRNA on migration of human colorectal cancer cell line LoVo
代新珍 1黄学平 2钟琳 2陈少红 2韩西群 1张玉 2朱梅刚 1赵彤1
作者信息
- 1. 南方医科大学,附属南方医院病理科,广东,广州,510515
- 2. 南方医科大学,基础医学院病理学系,广东,广州,510515
- 折叠
摘要
Abstract
AIM: To invesligale the expression of seplin 2 in human coloreclal cancer cell lines and the effecl of seplin 2 on the capacity of migration of human coloreclal cancer cell line LoVo. METHODS; Real — lime fluorescence quantitative RT — PCR and Western blolling analysis were used to determine the mRNA and protein levels of seplin 2 in different melaslalic potential cell lines, respectively. The expression of septin 2 in LoVo cells was silenced by siRNA. The efficacy of siRNA was confirmed by real — lime fluorescence quantitative RT — PCR. Seplin 2 and its co — localization with F — actin were measured by immunofluorescence melhod. The migration of Iransfecled cells was analyzed by scratch tesl. RESULTS; The expression of seplin 2 in LoVo cells was significantly higher than that in low melaslalic potential cell lines such as SW480, HCT — 116 and HT — 29 at both mRNA and protein levels. The mRNA expression of seplin 2 was successfully silenced in LoVo cells by siRNA. Cell wound closure rale was also decreased in seplin 2 siRNA group as compared wilh control group ( bolh P < 0. 05 ) . The co — expression of seplin 2 and F — aclin formed the typical filamentous — granular organization, and down — regulation of seplin 2 resulted in cell skeleton disturbance wilh less or shorter pseudopodium and decreased stress fibers. CONCLUSION; Seplin 2 is highly expressed in LoVo cells. Partial deletion of septin 2 represses the capability of tumor cell migralion.
关键词
Septin 2/LoVo细胞/细胞骨架Key words
Seplin 2/ LoVo cells/ Cell skeleton分类
医药卫生引用本文复制引用
代新珍,黄学平,钟琳,陈少红,韩西群,张玉,朱梅刚,赵彤..siRNA特异性沉默septin2基因抑制大肠癌LoVo细胞迁移[J].中国病理生理杂志,2012,28(8):1362-1366,5.
