中国病理生理杂志2012，Vol.28Issue(8)：1420-1423,4.DOI:10.3969/j.issn.1000-4718.2012.08.015
TGF-β1作用后老龄大鼠心肌成纤维细胞p38MAPK通路和JNK通路的变化
Age-associated changes of p38 MAPK and JNK signal pathways in cardiac fibroblasts treated with transforming growth factor beta 1
林运灵 1李维维 1陈良龙1
作者信息
- 1. 福建医科大学附属协和医院心内科,福建,福州,350001
- 折叠
摘要
Abstract
AIM: To invesligale the effect of aging on p38 milogen - aclivaled protein kinase ( MAPK) and c — Jun N — terminal kinase( JNK) signal pathways in ral cardiac fibroblasls ( CFs) . METHODS: Cardiac fibroblasls ob-Lained from neonalal and aged rals were cullured and randomly divided into 4 groups: neonalal PBS conlrol group ( N1 group ) , neonalal TGF - (3, trealmenl group ( N2 group) , aged PBS conlrol group ( A1 group) and aged TGF - β, trealmenl group (A2 group). Proliferation of CFs was delecled by MTT coloricmelric assay. The expression levels of tlotal p38 MAPK, JNK, phospho — p38 and phospho — JNK were measured by Weslern blolling. RESULTS; The proliferalive capacity of aged CFs was significantly decreased as compared with neonatal CFs after stimulated with TGF - (3,. In response to TGF - 1β , the expression levels of phospho - p38 and phospho - JNK were significantly increased in N2 group and A2 group as compared with Nl group and Al group, respectively. The levels of total p38 and nonphosphorylated JNK in N2 group were similar to those in A2 group. Compared with N2 group, the levels of phospho - p38 and phospho - JNK markedly decreased in A2 group. CONCLUSION: These data indicate that p38 MAPK and JNK signal pathways are impaired in aged CFs.
关键词
心肌成纤维细胞/转化生长因子β/p38 MAPK通路/JNK通路Key words
Cardiac fibroblasls/ Transforming growth faclor beta/ p38 MAPK pathway/ JNK pathway分类
医药卫生引用本文复制引用
林运灵,李维维,陈良龙..TGF-β1作用后老龄大鼠心肌成纤维细胞p38MAPK通路和JNK通路的变化[J].中国病理生理杂志,2012,28(8):1420-1423,4.
