中国病理生理杂志2012，Vol.28Issue(8)：1436-1440,5.DOI:10.3969/j.issn.1000-4718.2012.08.018
叶酸和维生素B12减轻高同型半胱氨酸血症诱导的老年大鼠阿尔茨海默样病变
Protective effect of folate and vitamin B12 on Alzheimer-like pathological changes in brain of aged rats with hyperhomocysteinemia
摘要
Abstract
AIM: To explore the effect of hyperhomocysleinemia on the Alzheimer — like pathological changes in the brain of aged rat. METHODS; The rats were Irealed with homocysleine (Hey) by inlravenous injeclion ihrough vena caudalis wilh or wilhoul a simultaneous supplementation of folale and Vitamin Bl2 (FB) in the drinking water for 28 weeks. The distribution and aggregation of tau protein were detected by Bielschowsky silver staining. Western blotting was used to determine the protein levels of tau phosphorylation, glycogen synthase kinase 3(3 ( GSK —3(3) and protein phospha-tase 2A (PP2A). RESULTS: Treatment with Hcy induced hyperhomocysteinemia in aged rats and resulted in the formation of neurofibrillary tangles in the brain. Supplementation of FB effectively reduced the tangles. Hyperhomocysteinemia also induced Alzheimer — like tau hyperphosphorylation at multiple sites (Ser199/202 and Ser396). Hyperhomocysteinemia activated GSK — 3(3 and inhibited the activity of PP2A. Supplementation of FB alleviated the changes of tau and the activities of GSK — 3(3 and PP2A. CONCLUSION: Supplementation of FB ameliorates the hyperhomocysteinemia — induced Alzheimer — like pathological changes of tau protein possibly through regulating the activity of GSK —3(3 and PP2A in aged rats.关键词
老年大鼠/Tau蛋白/叶酸/维生素B12/阿尔茨海默病Key words
Aged rat/ Tau protein/ Folic acid/ Vitamin B12/ Alzheimer disease分类
医药卫生引用本文复制引用
魏伟,陆大祥,戚仁斌,王达安..叶酸和维生素B12减轻高同型半胱氨酸血症诱导的老年大鼠阿尔茨海默样病变[J].中国病理生理杂志,2012,28(8):1436-1440,5.基金项目
国家自然科学基金资助项目 (No.81100944) （No.81100944）
广东省医学科研基金资助项目(No.B2011156) （No.B2011156）
暨南大学博士启动基金资助项目(No.21611334) （No.21611334）
