中国病理生理杂志2012，Vol.28Issue(8)：1441-1447,7.DOI:10.3969/j.issn.1000-4718.2012.08.019
新型大麻制剂O-1602和大麻二酚对DSS诱导的小鼠结肠炎的抗炎作用及其机制
Roles of novel cannabinoid chemicals O-1602 and cannabidiol in DSS-induced mouse colitis
摘要
Abstract
AIM: To invesligale the roles of 0 — 1602 and cannabidiol (CBD) in dexlran sulfale sodium (DSS) —induced mouse colilis. METHODS; The model of colilis was induced in C57BL/6 mice by drinking waler con-taining 4% DSS for 7 days. The model mice were trealed with 0 - 1602 (5 mg/kg) , CBD (1 mg/kg) or SB203580 [ an inhibitor of p38 milogen — aclivaled prolein kinase ( MAPK) at dose of 5 μmol/kg]. A colilis scoring syslem was used Lo e-valuale the colon local lesion, and the syslemic inflammalory responses were observed by delecling the plasma levels of Lumor necrosis faclor α ( TNF — α) , inlerleukin 6 (IL — 6 ) and cytokine — induced neulrophil chemoallraclanl 1 ( CINC — 1) , and the aclivily of myeloperoxidase ( MPO) in the lung tissues. The expression of G prolein — coupled receplor 55 ( GPR55 ) was delecled by the melhod of immunohislochemislry. The expression of p38 and phosphorylaled p38 ( p — p38 ) in colon lissues was determined by Weslern blolling. RESULTS: O — 1602 and CBD improved the pathological changes in the mice wilh DSS — induced colilis and decreased the plasma levels of TNF — α, IL — 6 and CINC — 1, and the aclivily of MPO in the lung lissues ( P < 0. 05 ) . Lower expression of p — p38 was observed after trealmenl wilh 0 — 1602, CBD and SB203580 (P <0. 05). The expression of GPR55 was mainly in the submucosa of mouse colon lissues. CONCLUSION: 0 —1602 and CBD show proleclive effecl on the mice wilh experimenlal colilis, and the anti — inflammalory roles of 0 — 1602 and CBD are relaled lo the inhibilion of p38 MAPK. The expression level of GPR55 in the submucosa of mouse colon lissue is low.关键词
炎症性肠病/大麻素类/p38 MAPK信号通路/G蛋白偶联受体55Key words
Inflammalory bowel disease/ Cannabinoids/ p38 MAPK signaling pathway/ G — protein — coupled receptor 55分类
医药卫生引用本文复制引用
冯雅静,李永渝,林旭红,李琨,余良英,曹明华,徐菁..新型大麻制剂O-1602和大麻二酚对DSS诱导的小鼠结肠炎的抗炎作用及其机制[J].中国病理生理杂志,2012,28(8):1441-1447,7.基金项目
国家自然科学基金资助项目(No.30971168 （）
No.81141050) （）
