中国病理生理杂志2012，Vol.28Issue(8)：1477-1481,5.DOI:10.3969/j.issn.1000-4718.2012.08.025
ERK信号通路在檞皮素促大鼠MSCs成骨分化中的作用
The roles of ERK signaling pathway in osteogenic differentiation of rat MSCs promoted by quercetin
摘要
Abstract
AIM: To study the roles of extracellular signal — regulaled kinase (ERK) signal pathway in the process of osteogenic differenlialion in rat mesenchymal stem cells (MSCs) promoted by quercetin (QUE). METHODS: The oplimal concenlralion of QUE for promoting osleogenic differentiation of ral MSCs was determined by MTT and alkaline phosphatase (ALP) deleclion. The activity of ALP was delecled by the ALP deleclion kit. The expression of bone Gla pro-Lein (BGP) and collagen Lype I (Col I ) was observed by ELISA analysis. MSCs were exposed Lo QUE at oplimal concenlralion with or wilhoul ERK1/2 inhibitor PD98059. Non — phosphorylaled and phosphorylaled expression of ERK1/2 was analyzed by Western blolling. The mRNA expression of transforming growlh faclor β1 ( TGF — β1 ) , bone morphogenelic prolein 2 (BMP —2) and core binding faclor α1 ( Cbfα1) was measured by fluorescence quanlilalive PCR. RESULTS: QUE al concentrations of 0. 1 μmol/L, 1 μmol/L and 10 μmol/L induced the expression of ALP in MSCs in a dose —dependent manner, and also promoled MSCs proliferation. The expression levels of ALP, BGP and Col I were higher in QUE group, and was lower in PD89059 group than those in conlrol group. Compared wilh conlrol group, the level of phosphorylaled ERK1/2, and the mRNA expression of TGF — β1 , BMP — 2 and Cbf α1 increased in QUE group. The mRNA expression of TGF- β1 , BMP -2 and Cbfα in QUE + PD98059 group decreased as compared with QUE group. CONCLUSION : QUE promotes osleogenic differentialion of MSCs by activating ERK signaling palhway.关键词
骨髓间充质干细胞/成骨分化/有丝分裂原活化蛋白激酶类/信号通路Key words
Bone marrow mesenchymal stem cells/ Osleogenic differenlialion/ Mitogen ?activated prolein ki-nases/ Signaling palhway分类
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奉水旺,杨丽,王攀攀,李淑琴,汪甜,尹素娟,张荣华..ERK信号通路在檞皮素促大鼠MSCs成骨分化中的作用[J].中国病理生理杂志,2012,28(8):1477-1481,5.基金项目
广东省教育厅育苗工程(No.34310016) （No.34310016）
