中国病理生理杂志2012，Vol.28Issue(8)：1482-1487,6.DOI:10.3969/j.issn.1000-4718.2012.08.026
Egr-1介导了IL-1β诱导的小鼠软骨细胞凋亡
Egr-1 mediated IL-1β-induced chondrocyte apoptosis in mice
摘要
Abstract
AIM: To invesligale the effect of early growth response prolein 1 ( Egr — 1) on inlerleukin — 1β (IL — 1 β ) — induced apoptosis of chondrocyles. METHODS: Mouse articular chondrocytes cultured in vitro were treated with IL — 1 β- Apoptosis was quantified by counting the cells with pyknotic nuclei after staining with Hoechst 33258. Quantitative RT — PCR was applied to detect the mRNA level of Egr — 1. The protein expression of caspase — 3 and Egr — 1 was detected by Western blotting. Chondrocytes were transfected with siRNA using Lipofectamine RNAiMAX. RESULTS; After treated with IL — 1β for4 h, 8 h and 12 h, the apoptotic rates of the cells were 7% , 22% and 41% , respectively, and up — regulation of cleaved caspase — 3 was observed. Up — regulation of Egr — 1 at mRNA and protein levels was induced by IL — 1β in a time — dependent manner. Knockdown of Egr — 1 expression decreased the 12 — hour rate of apoptosis from a-bout 40% to about 14% and 12% , and up — regulation of cleaved caspase — 3 induced by IL — 1β was inhibited. CONCLUSION : Egr — 1 is involved in the apoptosis of chondrocytes induced by IL — 1 β and may play an important role in the development of osteoarthritis.关键词
软骨细胞/细胞凋亡/早期生长反应蛋白1/白细胞介素1βKey words
Chondrocytes/ Apoptosis/ Early growth response protein 1/ Interleukin — 1 β分类
医药卫生引用本文复制引用
叶志强,张荣凯,陈郁鲜,戴海涛,冯辉,杜庚衡,曾花,曾春..Egr-1介导了IL-1β诱导的小鼠软骨细胞凋亡[J].中国病理生理杂志,2012,28(8):1482-1487,6.基金项目
广东省科技计划(No.2009A030200012 （）
No.2011B061300011) （）
