中国病理生理杂志2012，Vol.28Issue(8)：1494-1499,6.DOI:10.3969/j.issn.1000-4718.2012.08.028
哌仑西平对近视鸡眼巩膜基质MMP-2和TIMP-2表达的影响
Effects of pirenzepine on expression of MMP-2 and TIMP-2 in stroma of sclera in chick myopic eyes
摘要
Abstract
AIM: To observe the effect of inlravilreal injection of pirenzepine on form — deprivation myopia in chicks. METHODS: Forty — one day old chicks were randomly divided into 4 groups: normal control group, form deprivation group, vehicle control group and pirenzepine injection group. The right eyes of all chicks were used as experimental eyes. The deprived eyes in vehicle control group and pirenzepine injection group received daily intravitreal injection of vehicle control solution (0.01 mol/L PBS) and pirenzepine (l%in PBS) , respectively. Optical examinations such as refraction, axial length and equatorial diameter were made at the end of the 5 th day. The mRNA and protein levels of matrix met-alloproteinase 2 ( MMP —2) and tissue inhibitor of metalloproteinase —2 (TIMP — 2) , and the activity of MMP —2 were detected by RT — PCR, Western blotting and zymography analysis,respectively. RESULTS: Refraction, axial length and equatorial diameter of the eyes in pirenzepine injection group were significantly lower than those in form deprivation group and vehicle control group ( P < 0. 05 ) , but those were higher ( P < 0. 05 ) and the eyes were relatively myopic as compared with normal control group. The mRNA expression, protein levels and activity of MMP —2 in pirenzepine group were significantly higher than those in normal control group ( P < 0. 01) , and were significantly lower than those in form deprivation group and vehicle control group ( P < 0. 01, P < 0. 01). The mRNA and protein levels of TIMP — 2 in pirenzepine group were significantly lower than those in normal control group ( P < 0. 01) , and was significantly higher than those in form deprivation group and vehicle control group (P <0. 01, P <0. 01). CONCLUSION: Intravitreal injection of pirenzepine may partly pre-vent form — deprivation myopia by modulaling the expression of MMP — 2 and TIMP — 2 in the fibrous layer of solera.关键词
近视/哌仑西平/基质金属蛋白酶2/金属蛋白酶组织抑制剂2Key words
Myopia/ Pirenzepine/ Malrix metalloproleinase 2/ Tissue inhibitor of melalloproleinase - 2分类
医药卫生引用本文复制引用
戴淑真,张黎,王丽娅,曾骏文..哌仑西平对近视鸡眼巩膜基质MMP-2和TIMP-2表达的影响[J].中国病理生理杂志,2012,28(8):1494-1499,6.基金项目
美国CMB基金资助项目(No.98-677) （No.98-677）
广州市科技计划基金资助项目(No.2002z-0142) （No.2002z-0142）
