中国病理生理杂志2012，Vol.28Issue(8)：1500-1503,4.DOI:10.3969/j.issn.1000-4718.2012.08.029
废用状态下大鼠骨骼肌再负荷对比目鱼肌calpain活性和肌细胞膜通透性的影响
Effects of disuse and reloading on calpain activity and cell membrane permeability of soleus muscle fibers in rats
摘要
Abstract
AIM: To invesligale the effects of disuse and reloading on the calpain aclivily and cell membrane permeability of soleus muscle fibers in rals. METHODS; Tail — suspension (TS) rats were used as a muscular alrophy model. The female rals were randomly divided into conlrol group ( CON) , conlrol group with training ( CON + T) , 2 — week TS group (TS) and 2 — week TS wilh training group ( TS + T). The changes of cell membrane permeability and calpain aclivily were delecled wilh Evans blue (EB) as a tracer and casein zymography, respectively. RESULTS: The re-sults showed thal the EB was full of intercellular space in soleus muscle fibers in CON group, a few in TS group and much more in TS + T group, respectively. In TS group and TS + T group, some self — excilalion bands appeared and absorbance of calpain hydrolysis bands was significantly increased compared with those in the controls. CONCLUSION: The results suggest that the cell membrane permeability in TS + T group raises and the membrane barrier function is damaged. This may altribule to the hydrolysis of membrane skeleton proleins by calpain. Calpain — mediated accelerated degradation of cy-toskeleton proleins may be an importanl mechanism of skeletal muscle injury induced by reloading.关键词
萎缩/废用性/伊文思蓝/细胞膜通透性/钙蛋白酶/肌损伤Key words
Alrophy,disuse/ Evans blue/ Cell membrane permeability/ Calpain/ Muscle injury分类
生物科学引用本文复制引用
何越,高云芳,张少颖,张扬媚,孙小勇,王慧平..废用状态下大鼠骨骼肌再负荷对比目鱼肌calpain活性和肌细胞膜通透性的影响[J].中国病理生理杂志,2012,28(8):1500-1503,4.基金项目
国家自然科学基金资助项目(No.30770273) （No.30770273）
2011年度高等学校博士学科点专项科研基金资助课题(No.20116101110013) （No.20116101110013）
陕西省教育厅省级重点实验室重点科研计划项目 (No.11JS092) （No.11JS092）
