中国动脉硬化杂志2012，Vol.20Issue(8)：705-708,4.
阿托伐他汀对脂多糖诱导的人脐静脉内皮细胞肝X受体α及其靶基因表达的影响
Effect of Atorvastatin on mRNA Expression of Liver X Receptor α and Its Target Gene in Human Umbilical Vein Endothelial Cells Induced by Lipopolysacharide
顾文娟 1罗俊 1李江1
作者信息
- 1. 中南大学湘雅二医院心血管内科,湖南省长沙市410011
- 折叠
摘要
Abstract
Aim To investigate the effect of atorvastatin on mRNA expression of liver X receptor a (LXRa) and its target gene ATP-binding cassette transporter Al (ABCA1), sterol regulatory element binding protein-1 ( SREBP-1) in human umbilical vein endothelial cells (HUVEC) after treated by lipopolysacharide(LPS). Methods Human umbilical vein endothelial cells were cultured in vitro with gibco 1640 medium and 10 percent fetal bovine serum and 1 percent double antibiotics, and then were plated in 6-well plates at a denisity of approximately 2 X 10! cells per milliliter of media to be intervened. (1) Control group: HUVEC were cultured with the completed medium involving phosphate buffered saline (PBS), and LPS treated group or control intervention group: HUVEC were treated with LPS (the terminal concentration was 100 |ig/L) for 24 hours; (2) Atorvastatin intervention group or control intervention group; HUVEC were first treated with atorvastatin at different concentrations (0. 1, 1.0, 10.0 (unol/L) or dimethyl sulfoxide (DMSO) for 2 hours, and then co-treated with LPS for 22 hours. The level of LXRa and its target genes mRNA expression were measured by realtime polymerase chain reaction. Results Compared with control group, LPS could inhibit the mRNA expression of LXRa and its target genes ABCA1, SREBP-1 in HUVEC. The differences were statistically significant (P<0. 05). Compared with LPS plus DMSO group, atorvastatin could upregulate the mRNA expression of LXRa and its target gene mRNA expression in a dose-dependent manner. The differences were statistically significant (P <0. 05). Meanwhile, atorvastatin could downregulate the mRNA expression of inflammatory factor and adhesion factors in a dose-dependent manner.Conclusion These results demonstrate that LPS may inhibit the mRNA expression of LXRa and its target genes in HU-VEC, and atorvastatin can upregulate the mRNA expression of LXRa and its target gene mRNA expression in a dose-dependent manner to inhibit the inflammatory state of HUVEC.关键词
阿托伐他汀/人脐静脉内皮细胞/肝X受体αKey words
Atorvastatin/Human Umbilical Vein Endothelial Cells/Liver X Receptor a分类
医药卫生引用本文复制引用
顾文娟,罗俊,李江..阿托伐他汀对脂多糖诱导的人脐静脉内皮细胞肝X受体α及其靶基因表达的影响[J].中国动脉硬化杂志,2012,20(8):705-708,4.
