中国动脉硬化杂志2012，Vol.20Issue(9)：773-776,4.
肺炎支原体脂质相关膜蛋白在动脉粥样硬化发生发展中的作用机制
The Mechanisms of Mycoplasma Pneumoniae LAMP in the Development of Atherosclerosis
摘要
Abstract
Aim To study the proinflammatory cytokines production and apoptosis of macrophages upon Mycoplasma pneumoniae (M.pneumoniae) lipid-assorialed membrane proteins (LAMP) stimulation, and evaluate the molecular mechanism of M. pneumoniae to cause atherosclerosis. Methods The cultured human macrophages were incubated with various concentrations of M. pneumoniae LAMP preparations. The proinflammatory cytokines TNF-α, IL-β and 1L-6 production were detected by EL1SA and cell apoptosis by flow cytometry. At the same time, the activation of NF-kB and the effects of pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-kB , on M. pneumoniae LAMP-induced macrophages apoptosis were analyzed by Western blot. Results M. pneumoniae LAMP could induce high level of TNF-α, IL-1β and IL-6 production and macrophages apoptosis. These effects were highly associated with NF-kB activation of macrophages. PDTC could significantly inhibit the activation of NF-kB and partially reduce M. pneumoniae LAMP-induced macrophages secretion and apoptosis. Conclusions M. pneumoniae LAMP can induce proinflammatory cytokines secretion and macrophages apoptosis by activating NF-kB , and it may be an important etiological factor for atherosclerosis.关键词
肺炎支原体/脂质相关膜蛋白/前炎症因子/细胞凋亡/动脉粥样硬化Key words
Mycoplasma Pneumoniae/Upid- Associated Membrane Proteins/ Proinilammatory Cytokines/ Cell Apoptosis / Atherosclerosis分类
医药卫生引用本文复制引用
朱翠明,汪世平,游晓星,曾焱华,陈曦..肺炎支原体脂质相关膜蛋白在动脉粥样硬化发生发展中的作用机制[J].中国动脉硬化杂志,2012,20(9):773-776,4.基金项目
国家自然科学基金项目(81072418) （81072418）
湖南省教育厅优秀青年基金项目(09B088) （09B088）
湖南省高校科技创新团队资助项目(湘教通[2010]212号) （湘教通[2010]212号）
