中国动脉硬化杂志2012，Vol.20Issue(9)：792-796,5.
间歇性低氧通过上调脂蛋白相关磷脂酶A2和氧化型低密度脂蛋白表达促进动脉粥样硬化形成
Intermittent Hypoxia Promote the Formation of Atherosclerosis by Increasing Expression of Lipoprotein-Associated Phospholipase A2 and Oxidized Low Density Lipoprotein
李月春 1刘国荣 1王宝军 1郝喜娃 1张京芬 1庞江霞 1闫洁1
作者信息
- 1. 包头市中心医院神经内科,内蒙古包头市014040
- 折叠
摘要
Abstract
Aim To investigate the intermittent hypoxia and high-fat diet promoting the formation of atherosclerosis through increasing expression of lipoprotein-associated phospholipase A, (Lp-PLA,) and oxidized low density lipoprotein (ox-LDL). Methods Using randomized controlled study, prospective animal and factorial experiment, the animal models were established by intermittent hypoxia and high-fat diet. 24 New-Zealand white rabbits (4 months old) were divided into the following 4 groups; control group, intermittent hypoxia group(IH), high-fat-feeding group(HFD) and intermittent hypoxia & high-fat-feeding group (IH + HFD). Each group had 6 rabbits. Rabbits in IH group and IH + HFD group were placed in a cabin which nitrogen and air were periodically infused every 5 minutes for 8 h/day. The lowest level of oxygen concentration was at 8%. The highest level of oxygen concentration was at 21%. The HFD group and IH + HFD group were fed with fat-rich-stoyer. The intervention experiment lasted for 12 weeks. Blood samples for measurements of Lp-PLA; and ox-LDL were collected at 0 w, 4 w, 8 w and 12 w. The formation of atherosclerosis in the aortic arch and abdominal aorta was observed. Result The Lp-PLA2 level of IH group, HFD group and IH + HFD group al 8th and 12th week was higher than control group and at 4th week, the difference has statistic significance (P<0.05). The Lp-PLA, level of IH + HFD group at 4th, 8th and 12th week was higher than control group, IH group and HFD group) P < 0.05). The two factors of IH and HFD had significant syneTgistic effects on the Lp-PIA2 al 4th, 8ih and 12th week(f = 0.000,P =0.001 ,P =0.000). The ox-LDL level of IH group, HFD group and IH + HFD group at 4th, 8th and 12th week was higher than control group(P<0. 05). It was higher at 4th week than at 8th and 12th week (P<0.05). The ox-LDL level of IH + HFD group at 4th, 8th and 12th week was higher than control group, IH group and HFD group (P < 0.05). The two factors of IH and HFD had significant synergistic effects on the ox-LDL at 4th week( P = 0.000), however they had no significant synergistic effects at 8th and 12th week(P=0. 104 and P = 0. 166). The formation of atherosclerosis was observed by oil red " O" staining in abdominal aortic and by HE staining in aortic arch and abdominal aorta under IH and HFD intervention. Conclusion The intermittent hypoxia and high-fat diet can promote the formation of atherosclerosis through increasing expression of Lp-PLA2 and ox-LDL.关键词
间歇性低氧/高脂饮食/脂蛋白相关磷脂酶A2/氧化型低密度脂蛋白/动脉粥样硬化Key words
Intermittent Hypoxia/ High-fat diet/ Lipoprotein-Associated Phospholipase A2/ Oxidized Low Density Lipoprotein/ Atherosclerosis分类
医药卫生引用本文复制引用
李月春,刘国荣,王宝军,郝喜娃,张京芬,庞江霞,闫洁..间歇性低氧通过上调脂蛋白相关磷脂酶A2和氧化型低密度脂蛋白表达促进动脉粥样硬化形成[J].中国动脉硬化杂志,2012,20(9):792-796,5.
