中国康复2012，Vol.27Issue(3)：166-168,3.DOI:10.3870/zgkf.2012.03.002
溶血磷脂酸对小胶质细胞活化和吞噬功能的影响
Effect of LPA on activation and endocytosis of microglia
摘要
Abstract
Objectives To observe the effect of lysophosphatidic acid (LPA) on activation and endocytosis of microglia. Methods;BV2 cells in exponential phase were randomly divided into two groups: control group and LPA group. At time points (1 h and 2 h), cells were harvested for later use. FCM was used to detect the phagocytic rate of FITC-dextran and the fluorescence intensity. Results: As compared with control group, the phagocytic rate of FITC-dextran in LPA group was significantly increased at 1 h (P<0.05). At 2 h, the phagocytic rate of FITC-dextran reached the peak in both two groups. At time points (1 h and 2 h), as compared with control group, the fluorescence intensity in LPA group was increased (P<0. 01) and the product of phagocytic rate and fluorescence intensity in LPA group was significantly higher than that of control group (P<0. 01). Conclusion: LPA can promote activation and endocytosis of microglia in vitro, suggesting the roles of LPA in activation and endocytosis of microglia.关键词
溶血磷脂酸/小胶质细胞/FITC-葡聚糖/流式细胞术Key words
LPA/ microglia/ FITC-dextran/ FCM分类
医药卫生引用本文复制引用
付佩彩,喻志源,刘淼,唐荣华,王伟,骆翔..溶血磷脂酸对小胶质细胞活化和吞噬功能的影响[J].中国康复,2012,27(3):166-168,3.基金项目
国家自然科学基金项目(81000521,81030021) （81000521,81030021）
