中国临床药理学与治疗学2012，Vol.17Issue(4)：361-366,6.
大鼠原位肠肝血管灌流模型的优化及完善
Optimization of in situ perfused rat intestine-liver model
摘要
Abstract
AIM: To optimize perfusate composition of in situ perfused rat intestine-liver model and achieve the best perfusate formula. METHODS: The perfusion conditions were optimized t respectively. The Krebs-Ringer NaHCOs solution with bovine serum albumin and manni-tol and the Krebs-Ringer NaHCO3 solution with bovine serum albumin and dextran two T40 were contrasted for finding out the best perfusate medium. Then bovine serum albumin content in the optimal perfusion medium was optimized. RESULTS :K-R solution with bovine serum albumin and dextran T40 group was better than K-R solution with bovine serum albumin and mannitol group; 5% bovine serum albumin in perfusate is a more economic and ideal proportions. CONCLUSION: 5% bovine serum albumin in perfusate is a more economic and ideal proportions.关键词
大鼠原位肠肝血管灌流模型/右旋糖酐T-40/甘露醇/牛血清白蛋白Key words
In situ perfused rat intestine-liver model/ Dextran T40/ Mannitol/ Bovine serum albumin分类
医药卫生引用本文复制引用
王素军,莫李立,杨本坤,臧林泉,潘雪刁,王桂香,谭毓治,谢海棠..大鼠原位肠肝血管灌流模型的优化及完善[J].中国临床药理学与治疗学,2012,17(4):361-366,6.基金项目
国家自然科学基金(81073141) （81073141）
2010年科技部新药重大专项(2011zx09102-001-31) （2011zx09102-001-31）
广东省自然科学基金(9152402301000007) （9152402301000007）
