中国临床药理学与治疗学2012，Vol.17Issue(4)：367-370,4.
白细胞介素7剪接变异体的克隆和序列分析
Clone and analysis of splice variants of interleukin 7 in cancer cell lines
摘要
Abstract
AIM: To clone and select the splice variants in open reading frame of interleukin 7 from cancer cell lines. METHODS: Reverse transcription- polymerase chain reaction (RT- PCR) was used for investigating the expression of IL-7. According to IL-7 gene cDNA, the primers were designed and synthesized, then the splice variants of IL-7 in human cancer cell lines was identified, cloned into vector and se-quenced. RESULTS :IL 7 mRNA can be detected in Hepatic cancer cells and Colon cancer cells. A number of new bands of IL-7 were obtained from cultured cancer cell lines, which lack of different exons. By analyzing the splice variants, we found 5 of them in open reading frame of IL-7. CONCLUSION: Splice variants of IL-7 are expressed in many tumor cell lines. Further investigation of the Splice variants can provide a new research direction for cancer patient immune disorders and clinical immunotherapy.关键词
白细胞介素7/克隆/序列分析/肿瘤细胞/剪接体Key words
Interleukin 7/ Cloning/ Sequence analysis/ Tumor cells/ Splice variants分类
医药卫生引用本文复制引用
冯凤兰,彭羽瑜,潘德顺,李晓波,金小宝,朱家勇..白细胞介素7剪接变异体的克隆和序列分析[J].中国临床药理学与治疗学,2012,17(4):367-370,4.基金项目
中国-意大利科技合作项目(200625) （200625）
