中国临床药理学与治疗学2012，Vol.17Issue(4)：392-397,6.
热休克蛋白70肽段抑制胶原诱导关节炎小鼠Th17细胞生成
Heat shock protein 70 peptide inhibits Th17 cells in collagen-induced arthritis mice
摘要
Abstract
AIM: The effect of heat shock protein 70 (HSP70) peptide on Thl7 cells was studied in collagen-induced arthritis (CIA) mice model and the mechanism of its inhibitory effect on inflammation was also investigated. METHODS: DBA/1 mice were randomly divided into four groups including HSP70 peptide group, negative control group, PPD treated group and positive control group. Arthritis was induced by immunizing bovine type II collage, except negative control group. Pathology was evaluated by paw swelling and histological changes. The number of Thl7 cells in peripheral lymph nodes was assayed by flow cytometry, and Thl7 cell-associated regulatory factors were measured by real-time quantitative PCR. RESULTS. The number of Thl7 cells in HSP70 peptide treated group was significantly lower than that of positive control group and PPD treated group. Expression levels of IL-6, IL-23 , TGF-β1 and orphan nuclear receptor (RORγt) in HSP70 peptide group were markedly reduced than those of positive group and PPD group. CONCLUSION: HSP70 peptide could play a protective role on CIA mice through decreasing Thl7 cell regulating factors and inhibiting the differentiation of Thl7 cells.关键词
HSP70肽段/Th17细胞/CIA小鼠Key words
Heat shock protein 70/ T helper 17 cells/ Collagen-induced arthritis mice分类
医药卫生引用本文复制引用
郭鸿雁,袁慧慧,张须龙,赵文明..热休克蛋白70肽段抑制胶原诱导关节炎小鼠Th17细胞生成[J].中国临床药理学与治疗学,2012,17(4):392-397,6.基金项目
国家自然科学基金(30873185) （30873185）
973计划项目(2010CB529106)资助 （2010CB529106）
