中国临床药理学与治疗学2012，Vol.17Issue(6)：609-615,7.
表没食子儿茶素没食子酸酯对Reh细胞增殖及RUNX3基因甲基化状态的影响
Effects of Epigallocatechin-3-galate on growth inhibition of Reh cellline and methylation of RUNX3 gene
摘要
Abstract
AIM: To investigate the effects of epigallocatechin-3-gallate on the proliferation and the methylation status of Reh cells, and to explore the mechanism of the silencing of tumor suppressor gene RUNX3 and the regulating effect of EGCG on RUNX3 gene expression. METHODS: The cultured Reh cells were incubated with EGCG for different concentrations of 5, 10, 15 and 20 μg/mL, MTT assay was used to analyse the inhibition of cell proliferation by EGCGt cell apoptosis index was examined by flow cytometry, methylation-specific PCR (MSP) analysis was evaluated for the promoter region methylation status of the RUNX3 gene in Reh cell line. The mRNA and protein level of RUNX3 was detected by RT-PCR and western blot. RESULTS: EGCG of different concentrations could significantly inhibit proliferation and increase the apoptosis index of Reh cells in dose-and-time dependent manners. After treated with EGCG. the promoter region methylation status of the RUNX3 gene representing partial demeth-ylation could be detected in Reh cells. RT-PCR and western blot also showed the mRNA level of RUNX3 was increased in dose-and-time dependent manner. CONCLUSION- EGCG could depress the proliferation rate of Reh. The possible mechanism might be its reversing the hyperm-ethylation of RUNX3 gene and activiting the expression of RUNX3 gene.关键词
表没食子儿茶素没食子酸酯/RUNX3/甲基化Key words
Epigallocatechin-3-gallate/RUNX3/ Methylation分类
医药卫生引用本文复制引用
吴明彩,韦中玲,蒋明,方基勇,毕富勇..表没食子儿茶素没食子酸酯对Reh细胞增殖及RUNX3基因甲基化状态的影响[J].中国临床药理学与治疗学,2012,17(6):609-615,7.基金项目
安徽高校省级自然科学研究项目(KJ2010B249) （KJ2010B249）
