中国美容医学2012，Vol.21Issue(7)：1159-1162,4.
微针技术结合乙醇脂质体促进重组人酸性成纤维细胞生长因子透皮吸收的研究
Study on combination stratege of microneedles array technology and ethosomes for improving the permeation rate of rhaFGF through skin
冯苏云 1范金财 1刘立强 1田佳 1甘承 1杨增杰 1付思祺 1陈文霖1
作者信息
- 1. 北京协和医学院·中国医学科学院·整形外科医院整形九科 北京100144
- 折叠
摘要
Abstract
Objective To explore a minimally invasive administration method of rhaFGF to promote the permeation rate through skin. Methods Following treatment of microneedle arrays in skin of rabbit.the distribution and the depth of microporous were visualized using methylene blue staining of the skin and HE staining of cryostat sections.Different groups: microneedle with blank liposome group,microneedle with rhaFGF direct coating group,microneedle with rhaFGF ethosomes direct coating group, intradermal injection group were applied in this research.Distribution of rhaFGF was confirmed by immunofluorescence and immunohistochemical study, rhaFGF concentration of the skin homogenate of rabbit graft mode were analyzed using ELISA. Results After treatment of microneedle arrays 30min later of administration.the homogenate of skin tissue showed a significant (P<0.05) increase in concentration of microneedle with blank liposome group compared with rhaFGF direct coating group,Microneedle with rhaFGF ethosomes direct coating group.intradermal injection group compared with the rhaFGF direct coating group.Changes between microneedle with rhaFGF direct coating group,microneedle with rhaFGF ethosomes direct coating group showed a significant (P<0.05). Changes between Microneedle with rhaFGF ethosomes direct coating group, intradermal injection group showed no significant (P>0.05).Immunofluorescence and immunohistochemical staining of skin for rhaFGF showed that a large number of rhaFGF were arranged equality in dermal tissue of the microneedle with rhaFGF ethosomes direct coating group. Conclusion Combinating ethosomes with microneedles array technology can get a higher effection through the skin for rhaFGF. It make rhaFGF promote skin proliferation.accelerate skin flap expansion by transdermal delivery systerm becomes possible.They were easy and painless.lt maybe a new way to improve the absorption through skin for the macromolecular medicine such as polypeptide and protein关键词
重组人酸性成纤维细胞生长因子/乙醇脂质体/微针阵列/经皮给药Key words
rhaFGF/ethosomes/microneedle array/transdermal delivery分类
生物科学引用本文复制引用
冯苏云,范金财,刘立强,田佳,甘承,杨增杰,付思祺,陈文霖..微针技术结合乙醇脂质体促进重组人酸性成纤维细胞生长因子透皮吸收的研究[J].中国美容医学,2012,21(7):1159-1162,4.
