中国农业科学2012，Vol.45Issue(9)：1676-1684,9.DOI:10.3864/j.issn.0578-1752.2012.09.002
一个玉米细胞质雄性不育系的鉴定及遗传分析
Identification and Genetic Analysis of a Cytoplasmic Male Sterile Line in Maize (Zea mavs L.)
摘要
Abstract
[Objective] A new cytoplasmic male sterile line (Nongxi 928cms-Q1261) was identified for type of male sterile, stage of microspore abortion and genetic mechanism for its application to maize breeding. [Method] The male sterile expression was observed by planting sterile line in different locations and years. Testcross, sister cross and reciprocal cross were conducted with Nongxi 928cms-Q1261 to analyze the genetic characteristics of its male sterility. Meanwhile, the cytoplasmic type was discriminated by PCR amplification with specifically primers and the microspore genesis was observed under optical microscope by paraffin section technique. [Result] Results showed that the male sterility characteristics of Nongxi 928cms-Q1261 were stable, no anthers exposed on tassel and pollen aborted completely. The cytoplasmic male sterile gene, which was classified into S type of CMS, originated from Q1261, and its nuclear sterile gene originated from Nongxi 928, which was controlled by a pair of recessive genes. The microspores started degrading at the late stage of microspore and degenerated completely at the mature pollen stage. The male sterility of Nongxi 928cms-Q1261 could be maintained by inbred line Zheng58. [Conclusion] Nongxi 928cms-Q1261 belongs to S-type of CMS and is a non-pollen-type cytoplasmic male sterile line with many restorer lines.关键词
玉米/细胞质雄性不育/育性鉴定/遗传分析/胞质分类Key words
maize/cytoplasmic male sterile/fertility identification/genetic analysis/cytoplasm classification引用本文复制引用
祝丽英,陈景堂,黄亚群,赵永锋,宋占权..一个玉米细胞质雄性不育系的鉴定及遗传分析[J].中国农业科学,2012,45(9):1676-1684,9.基金项目
国家"863"重点课题(2011AA10A103-3) （2011AA10A103-3）
国家"十二五"科技支撑计划(2011BAD35B01) （2011BAD35B01）
