中国农业科学2012，Vol.45Issue(9)：1714-1722,9.DOI:10.3864/j.issn.0578-1752.2012.09.006
高粱品种萌发期耐盐性筛选与鉴定
Screening and Identification of Sorghum Cultivars for Salinity Tolerance During Germination
摘要
Abstract
[Objective] The objectives of the current study were to explore the response of different sorghum cultivars to salt stress, screen sorghum cultivars suitable for growing in saline soils, and provide a foundation for management of salt stress. [Method] Thirty seeds of each of 42 sorghum cultivars were treated with 150 mmolL"'NaCl or distilled water and incubated in Petri dishes for 10 days at humidity of 60%, light/dark of I2h/12h, illumination of 34 umolmV and 281C/25'C of day/night. Germination and growth were assessed by counting the number of radicals and coleoptiles and leaves that had appeared on a daily basis from the fourth day and measuring their lengths and dry weights on the tenth day using destructive sampling. The cultivars were classified on the basis of salinity tolerance by means of principal component analysis and cluster analysis considering the range of data on germination and growth traits. [Result] The correlation analysis on the relative values of most germination and growth parameters showed positive relationships among several variables. The cultivars were ranked based on the principal components analysis and five groups on salinity tolerance were sorted for the 42 sorghum cultivars according to the cluster analysis.[Conclusion] Principal component analysis results showed that radical length, coleoptiles and leaf weight and germination rate were the most significant factors and are recommended as the main indexes to identify salinity tolerance of sorghum at germination. The analysis showed five of the 42 cultivars were highly salt tolerant, e.g. Liaoza 15, fourteen cultivars were salt tolerant, e.g. Shenshi 104, twelvecultivars were medium salt sensitive, e.g. Aoza 1, eight cultivars were salt sensitive, e.g Tieza 17, and three were highly salt sensitive, e.g. Longza 10. As this research only relates to ranking sensitivity during germination, further work is needed to determine the mechanism of tolerance and its persistence through the plant life cycle.关键词
高粱/NaCl胁迫/耐盐性/主成分分析/聚类分析Key words
sorghum/NaCI stress/salinity tolerance/principal components analysis/cluster analysis引用本文复制引用
孙璐,周宇飞,汪澈,肖木辑,陶冶,许文娟,黄瑞冬..高粱品种萌发期耐盐性筛选与鉴定[J].中国农业科学,2012,45(9):1714-1722,9.基金项目
农业部现代农业产业技术体系建设专项资金项目(CAR-06-02-02) （CAR-06-02-02）
