中国农业科学2012，Vol.45Issue(9)：1881-1886,6.DOI:10.3864/j.issn.0578-1752.2012.09.025
乳酸菌对Caco-2细胞分泌APRIL和IL-10的影响
Effects of Lactic Acid Bacteria on APRIL and IL-10 Secretion in Caco-2 Cells
摘要
Abstract
[Objective] The study was designed to evaluate the immunological effects of probiotic strains Enterococcus faecium and Lactococcus lactis on production of pro-inflammatory cytokine of APRIL and anti-inflammatory cytokine interleukin-10 in intestinal epithelial cells. [Method] Human colon adenocarcinoma cell line, Caco-2 cells were incubated with PBS (Group CT, negative control), Escherichia coli K88 (Group EC, positive control), and Enterococcus faecium (Group EF), and Lactococcus lactis (Group LL) for 2 h, respectively. Besides, Caco-2 cells were firstly treated with E. Faecium and L. Lactis for 1 h, respectively, and then followed by incubation with E. Coli K.88 for an additional 2 h (Group EF-EC and Group LL-EC). Culture supematants were collected for analyzing the contents ofAPRIL and IL-10 by ELISA method. [Result] Results showed that E.faecium and L. Lactis induced an increased release ofAPRIL and IL-10. Pre-culture of Caco-2 cells with E. Faecium and L. Lactis was capable of markedly up-regulating production of IL-10 while decreasing APRIL secretion following co-culture with E. Coli K.88. [Conclusion] These findings demonstrated that E. Faecium and L. Lactis could activate immunity of intestinal epithelial cells by inducing APRIL and IL-10 secretion. Moreover, these two strains of lactic acid bacteria also exhibited anti-inflammatory properties via modulating the immune response in Caco-2 cells when they were infected with E. Coli K88.关键词
Caco-2细胞/屎肠球菌/乳酸乳球菌/大肠杆菌K88/APRIL/IL-10/ELISAKey words
Caco-2 cell/Enterococcus faecium/Lactococcus lactis/Escherichia coli K88/APRIL/IL-10/ELISA引用本文复制引用
黄怡,黄琴,李雅丽,崔志文,李卫芬,余东游..乳酸菌对Caco-2细胞分泌APRIL和IL-10的影响[J].中国农业科学,2012,45(9):1881-1886,6.基金项目
浙江省重大科技专项(2006C12086) （2006C12086）
国家重点基础研究发展计划(2009CB118705) （2009CB118705）
博士学科点专项科研基金(20110101110101) （20110101110101）
