中国农业科学2012，Vol.45Issue(12)：2346-2356,11.DOI:10.3864/j.issn.0578-1752.2012.12.002
大豆二粒荚长、宽相关QTL间上位效应和QE互作效应分析
Epistatic Effects and QE Interaction Effects of QTLs for Two-Seed Pod Length and Width in Soybean
摘要
Abstract
[ Objective ]The objective of this study was to investigate the epistatic effects and QE interaction effects of QTLs for two-seed pod length and two-seed pod width. [Method] In order to find out these results, a F2:14-F2:1g RIL population containing 147 lines derived from a cross between Charleston as female parent and Dongnong594 as male parent were used in this experiment. A genetic linkage map was constructed with 164 SSR primers screened in two parents and amplified in 147 lines population. QTLs and epistatic effects and QE Interaction effects of QTLs were located on one site in five years with a mixed linear model (QTLmapper1.6) . QTLs for these were detected in a five-year experiment with the recombination inbred lines (RIL) population derived from a cross between Charleston and Dongnong 594. [Result] Eight two-seed pod length QTLs and six two-seed pod width with additive effects were detected, the additive effects contribution rate were 27,2% and 16.27%, respectively, the general contribution of interaction between QTLs and environment were 10.19% and 12.18%. Nine pairs of two-seed pod length QTLs with epistatic effects were found in the RIL, accounting for 9.02% of the general phenotypic variation, and eight pairs of two-seed pod width QTLs accounting for 8.81% of the general phenotypic variation.[Conclusion]The results indicated that the epistatic effects and the environmental factor played an important role in the inheritance of two seed-pod length and width in soybean. Therefore, both major and minor QTLs should be considered in the improvement in soybean breeding.关键词
大豆/二粒荚长/二粒荚宽/混合线性模型/QTL与环境互作效应/上位互作效应Key words
soybean/ two-seed pod length/ two-seed pod width/ mixed linear model/ QTL× environment interaction/ epistatic effects引用本文复制引用
杨振,裴宇峰,谢圣男,刘春燕,蒋洪蔚,韩雪,辛大伟,陈庆山,胡国华..大豆二粒荚长、宽相关QTL间上位效应和QE互作效应分析[J].中国农业科学,2012,45(12):2346-2356,11.基金项目
“大豆新品种培育与扩繁”国家十二五科技支撑计划(2011BAD35B06)、农业部转基因专项(2011ZX08004-001-06)、现代农业产业体系(CARS-04-02A)、行业科技——气候变化对大豆生产的影响及应对技术研究(200903003) （2011BAD35B06）
