中国农业科学2012，Vol.45Issue(12)：2404-2411,8.DOI:10.3864/j.issn.0578-1752.2012.12.008
刀孢蜡蚧菌的鉴定及其对南方根结线虫不同生活阶段的定殖
Identification of Fungus Lecanicillium psalliotae and Its Colonization in Different Life Stages of Meloidogyne incognita
摘要
Abstract
[Objective] The objective of this study is to investigate the control potential of egg-parasitic fungi Lecanicillium psalliotae against root-knot nematodes as an useful biological control agent. [ Method ] The fungus strain CGMCC5329 was isolated from eggs of Meloidogyne incognita infecting sponge gourd in Chengyang, Qingdao, Shandong Province. Based on morphological characters and molecular analysis of ITS-rDNA, the strain was identified as L. psalliotae. Colonization of L. psalliotae in different life stages of M. incognita was observed by microscope. [Result] The fungus colonized in different life stages of M. incognita including eggs, juveniles and females, and demonstrated the great suppression in hatch of egg masses. The infection pegs and fungal networks on egg were observed on surface of infected eggs which showed a wrinkled and shrunken appearance. The embryonic development was arrested by the fungal invasion and high vacuolation was also observed. In some cases the conidiating hyphae were also seen outside the egg surface. The egg parasiting rate by fungi was 85.76% on 6th day. The inhibition in hatch of egg masses was 91.41% on 15th day. The 2nd stage juveniles were penetrated with networks of hyphae and the body were full of fungal hyphae. The juvenile parasitic rate by fungi was 79.23% on 5th day. The extensive network and infection pegs were formed on females parasited with fungi which resulted in body deformation, sometimes the contents extravasated. [Conclusion] L. psalliotae CGMCC5329 not only colonizes the sedentary females and eggs, but also infects 2nd stage juveniles. It could be used as an useful biological control agent against root knot nematodes.关键词
刀孢蜡蚧菌/南方根结线虫/ITS序列/系统发育/致病性Key words
Lecanicillium psalliotae/ Meloidogyne incognita/ ITS sequence/ phylogeny/ pathogenicity引用本文复制引用
曹君正,武侠,林森..刀孢蜡蚧菌的鉴定及其对南方根结线虫不同生活阶段的定殖[J].中国农业科学,2012,45(12):2404-2411,8.基金项目
山东省自然科学基金项目(ZR2010CM037)、农业部科研项目(2130108)、山东省“泰山学者”建设工程专项经费、中国烟草总公司项目(110200902065-4) （ZR2010CM037）
