中国农业科学2012，Vol.45Issue(12)：2462-2467,6.DOI:10.3864/j.issn.0578-1752.2012.12.015
褪黑激素对Bcl-2和Bax基因在绒山羊皮肤中表达的影响
Effects of Melatonin on the Expression of Bcl-2 and Bax Genes in Skin of Cashmere Goat
摘要
Abstract
[ Objective ] The present study was aimed to research the effects of melatonin on the expression of Bcl-2 and Bax genes in the skin of Inner Mongolia Cashmere goat, then discuss the relationships between Bcl-2, Bax, and melatonin and the growth and apoptosis of cashmere goat. [Method] Eight Inner Mongolia Cashmere goats, with the same age, sex and liveweight were chosen. Four of them were treated with melatonin, the rest were used as control. Total RNA of skin was extracted, and expression of Bcl-2 and Bax genes were analyzed by real-time PCR. [Result] The changing characteristics of Bcl-2/Bax coincided with the growth rhythm of cashmere of Inner Mongolia Cashmere goat. Melatonin had no significant effects on expression of Bcl-2 gene (P>0.05), but significantly up-regulated the Bax gene (P<0.01). [Conclusion] Melatonin down-regulated the ratio of Bcl-2/Bax in the skin of Inner Mongolia Cashmere goat, which promoted the apoptosis and accelerated the metabolism.关键词
绒山羊/褪黑激素/Bcl-2基因/Bax基因Key words
cashmere goat/ melatonin/ Bcl-2 gene/ Bax gene引用本文复制引用
刘慧,冯林,王瑞军,张燕军,李金泉,张文广,王志新,张永斌,何云梅,蒋再平,丽春..褪黑激素对Bcl-2和Bax基因在绒山羊皮肤中表达的影响[J].中国农业科学,2012,45(12):2462-2467,6.基金项目
现代农业产业技术体系建设专项资金(CARS-40)、公益性行业(农业)科研专项(201003019)、国家科技支撑计划项目(2011BAD28B05)、国家自然科学基金项目(30960246) （CARS-40）
