中国农业科学2012，Vol.45Issue(12)：2482-2490,9.DOI:10.3864/i.issn.0578-1752.2012.12.018
斑鸠对H9N2亚型禽流感病毒的易感性及其流感病毒受体类型的研究
Study on the Susceptibility of Turtledoves to Avian Influenza Virus Subtype H9N2 and its Receptor
摘要
Abstract
[Objective] The susceptibility of turtledove to H9N2 subtype avian influenza virus and the type and distribution of influenza virus receptors in the respiratory tract epithelium of turtledove were studied. [Method] Eight turtledoves and eight SPF chickens were inoculated with A/Chicken/Beijing/2/2009 H9N2 via oculonasal routes at a dose of 5× 104ElD50 per bird. In addition to observation of clinical signs and gross pathological changes and histopathological changes, the location of the viral antigen and antibody detection were used. Influenza A matrix protein was observed by immunohistochemistry straining. The type of SAa2, 6Gal and SAo2, 3Gal linkage on these animals' trachea and larynx epithelium cells were detected by digoxigenin-labeled lectin staining. [Result] On day 5 post inoculation, no abnomal clinical signs and pathological changes were found in the turtledoves and SPF chickens. The epithelium was found with different degrees of swelling in the turtledove' larynx , upper trachea, middle trachea and lower trachea. The virus positive particles were detected in turtledoves' upper trachea, middle trachea , lower trachea and lung epithelium with the method of immunohistochemistry; H9N2 A1V was isolated from turtledoves, and the positive rate of turtledoves' nasopharyngeal swab was 75% (6/8), that of SPF chickens' was 100% (8/8). On 14th day post inoculation, HJ antibody was detected, and the positive rate of turtledoves' was 80% (4/5), that of SPF chickens was 100 (5/5). SAα2, 3Gal and SAa2. 6Gal were both detected on turtledove's larynx, upper trachea, middle trachea and lower trachea [ Conclusion ] The turtledoves are susceptible to A/Chicken/Beijing/2/2009, and there are both SAα2, 3Gal binding avian influenza virus and SAα2, 6Gal binding human influenza virus on turtledove's larynx , upper trachea, middle trachea and lower trachea.关键词
斑鸠/H9N2亚型禽流感病毒/流感病毒受体/易感性Key words
turtledove/ H9N2 Subtype avian influenza virus/ influenza virus receptor/ susceptibility引用本文复制引用
丁佩佩,刘月焕,陈明勇,韩春华,林健,韩婧雯,潘洁..斑鸠对H9N2亚型禽流感病毒的易感性及其流感病毒受体类型的研究[J].中国农业科学,2012,45(12):2482-2490,9.基金项目
国家自然科学基金项目(NO.31072159) （NO.31072159）
