中国水产科学2012，Vol.19Issue(4)：577-585,9.DOI:10.3724/SP.J.1118.2012.00577
基于SSU rDNA和线粒体cox1序列分析甲藻中不同生态类群的演化规律
Analysis of the evolution of different ecological groups of dinoflagellates based on SSU rDNA and mitochondrial cox 1 sequences
摘要
Abstract
In this paper, we analyze the evolution of different ecological groups of dinoflagellates, based on SSU rDNA and mitochondrial cox 1 sequences. After PCR and sequencing, we obtained partial sequences of SSU rDNA and mitochondrial cox 1 for 4 dinoflagellates. By combining with related sequences of 26 dinoflagellates downloaded from GenBank, their maximum likelihood (ML) and neighbor-joining trees were constructed, using Plasmodium falciparum as the outgroup. The tree branch structure was evaluated by bootstrap support and calculation of the posterior probability was employed to assess the overall tree structure. This was followed by assessment of the two ML tree topologies by lsKH, SH, ELW and 2sKH tests. This analysis indicated that benthic Pro-rocentrum did not cluster with planktonic Prorocentrum in phylogenetic trees based on different genes. Analysis of Amphidinium carterae further supported their unique evolutionary positions. We conclude that the phylogenetic tree based on SSU rDNA and mitochondrial cox 1 substantially reflects the evolution of dinoflagellates. The results of this study laid a foundation for further research on the evolution between different ecological groups of dinoflagellate from the genetic level.关键词
浮游型甲藻/底柄型甲藻/利玛原甲藻/强壮前沟藻/SSU rDNA/cox1Key words
Planktonic dinoflagellates/ benthic dinoflagellates/ Prorocentrum lima/ Amphidinium carterae/ SSU rDNA/ cox 1分类
农业科技引用本文复制引用
唐晨,董丽,饶涛,贾睿,于克锋,吴维宁,王金辉,徐韧,何培民..基于SSU rDNA和线粒体cox1序列分析甲藻中不同生态类群的演化规律[J].中国水产科学,2012,19(4):577-585,9.基金项目
上海市优秀学科带头人计划项目(08XD1403700) （08XD1403700）
上海市科委国际合作项目(08540702600) （08540702600）
上海市科委农业重点攻关项目(10391901900) （10391901900）
海洋公益性行业科研专项经费(200905010) （200905010）
国家海洋局重点实验室开放研究基金资助项目(MATHAB200907). （MATHAB200907）
