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格氏线虫表皮蛋白和分泌蛋白抑制昆虫免疫活性的比较

刘劲 刘华 袁京京 刘峥 杨秀芬 张利莉 邱德文 曾洪梅

中国生物防治学报2012,Vol.28Issue(3):400-407,8.
中国生物防治学报2012,Vol.28Issue(3):400-407,8.

格氏线虫表皮蛋白和分泌蛋白抑制昆虫免疫活性的比较

Comparison of the Immunosuppressive Activity between Cuticle Coat Proteins and Secrete Proteins from Steinernema glaseri

刘劲 1刘华 2袁京京 2刘峥 2杨秀芬 2张利莉 3邱德文 2曾洪梅2

作者信息

  • 1. 塔里木大学生命科学学院/新疆建设兵团塔里木盆地生物资源保护与利用重点实验室,阿拉尔843300 中国农业科学院植物保护研究所/农业部作物有害生物综合治理重点实验室,北京100081
  • 2. 中国农业科学院植物保护研究所/农业部作物有害生物综合治理重点实验室,北京100081
  • 3. 塔里木大学生命科学学院/新疆建设兵团塔里木盆地生物资源保护与利用重点实验室,阿拉尔843300
  • 折叠

摘要

Abstract

Both surface coat proteins (SCPs) and secrete proteins (SPs) from Steinernema glaseri infective juveniles are able to suppress insect immune activities and were proved to play important roles in promoting infection of entomopathogenic nematodes. However, they are different in activities because of their different constituents. In this study, the SCPs were extracted with ethanol and the SPs were induced by Galleria me[lonella homogenates, then the similarity and difference between them were compared. Result indicated that no matter in vivo or in vitro, both kinds of proteins have the ability to suppress insect immunity, but the activity of SPs is more powerful than that of SCPs. Our studies provide scientific evidence for further study of the relationship between entomopathogenic nematode and insect, and the immunosuppressive mechanism of Steinernema glaseri.

关键词

格氏线虫/表皮蛋白/分泌蛋白/血细胞裂解/抗吞噬作用

Key words

Steinernema glaseri/surface coat proteins/secrete proteins/lysis of hemocyte/antiphagocyticactivity

分类

农业科技

引用本文复制引用

刘劲,刘华,袁京京,刘峥,杨秀芬,张利莉,邱德文,曾洪梅..格氏线虫表皮蛋白和分泌蛋白抑制昆虫免疫活性的比较[J].中国生物防治学报,2012,28(3):400-407,8.

基金项目

国家自然科学基金 ()

948项目 ()

中国生物防治学报

OA北大核心CSCDCSTPCD

2095-039X

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