中国生物防治学报2012，Vol.28Issue(3)：415-423,9.
禾长蠕孢菌稗草专化型固体发酵产孢培养基及其工艺条件的研究
The Medium and Culture Conditions ofHelminthosporium gramineum Rabenh. f. sp. echinochaloae with Solid State Fermentation
摘要
Abstract
Helminthosporium gramineum Rabenh f. sp. echinochloae （HGE） is a potential biocontrol agent for bamyardgrass control in rice field. Authors optimized the medium and culture conditions in a solid state fermentation system for mass production ofHGE conidia. Investigation on carbon source, nitrogen source and solid substrate indicated that a medium consisted of 4% rice flour, 1% soybean meal, 0.2% Na3PO4·12H2O, 0.1% MgSO4·7H2O and pedite was better for high yield of condia. 40% medium moisture, 8% inoculum concentration, 25℃ and 11 d incubation were good for the conidial production. By the medium and culture conditions, the maximum conidial yield was 1.5×107 conidia.g-1 dry material. Pot bioassay in the greenhouse gave rise to 80% control efficacy against barnyardgrass after treatment with HGE conidia at 0.6 g-m-2.关键词
禾长蠕孢菌稗草专化型/固体发酵/产孢/稗草生物防治Key words
Helminthosporium gramineum Rabenh f. sp. echinochloae/solid-state fermentation/conidialproduction/barnyardgrass/biological control分类
农业科技引用本文复制引用
段桂芳,杨爽,张建萍,余柳青..禾长蠕孢菌稗草专化型固体发酵产孢培养基及其工艺条件的研究[J].中国生物防治学报,2012,28(3):415-423,9.基金项目
浙江省重大项目 （）
浙江省农业“生物三药”产业创新团队项目 （）
