中国输血杂志2012，Vol.25Issue(5)：442-445,4.
新型光敏剂MO07剂量及光剂量与红细胞溶血率的相关性研究
Dose-related injury on red blood cells caused by treatment of M007-mediated photochemical
吴文知 1黄毅 2王艳萍 3杨春晖 2张兰3
作者信息
- 1. 四川省骨科医院,四川成都,610041
- 2. 中国医学科学院北京协和医学院输血研究所
- 3. 四川大学华西医院肿瘤分子诊断研究室
- 折叠
摘要
Abstract
Objective To study the M007-mediated photodynamic inactivation be used to inactive tumor cells in RBC reclaimed in cancer surgery. Methods One experiment group and three control groups were eet: 1) Experiment group (M007-PCT): RBC plus M007 with irradiation;2) Pbotosensitizer group (M007): RBC plus M007 with no light; 3) Irradiation group (L) : RBC with irradiation;4) Control group (C) : with RBC only. The drug dose and light energy density ranging between 4 and 40μmol/L, and from 2. 16 to 6.48 J/cm2, respectively, were varied. RBC hemolvsis rate were e-valuated following these treatments. Results RBC of group M007-PCT and Group M007 hemolysiaed at the light dose of 2.16 J/cm2. M007 dose ranged from 4 to 40 μmol/L, the RBC hemolysis rates of group M007-PCT and group M007 increased from (0.108 ±0.003)%, (0.092 ±0.001)% to( 1.032±0.074)%,(l. 022 ±0.053)% respectively, dramatically lager than that of group L and C (P < 0.05 ). The light dose ranged from 2.16 J/cm2 to 6.48 J/cm2, hemolysis rate of group M007-PCT (28, 32, 36, 40ujnol/L) increased from (0.920 ± 0. 042)% 、(0.945 ± 0. 054)% 、(1.035 ± 0.052)%,(1.032 ±0.074)% to (1.489 ±0.315)% ,(1.506 ±0.110)% 、( 1.484 ±0. 308)% ,(1.502 ±0.264)%. Conclusion The level of M007-PCT response and the RBC hemolysis rate shows a strong dependence on the drug dose and light energy density in the applied conditions. RBC hemolysis slightly occurred in samples plus M007 without red light, showing dependence on the drug dose too (r = 0.956 、r = 0.946). The dose of experiment group is less than 1.7% hemoly-sis rate.关键词
光化学反应/光敏剂/光剂量/溶血率Key words
Photochemical action/Photosensitizer/Light dose/Hemolysis分类
临床医学引用本文复制引用
吴文知,黄毅,王艳萍,杨春晖,张兰..新型光敏剂MO07剂量及光剂量与红细胞溶血率的相关性研究[J].中国输血杂志,2012,25(5):442-445,4.
