中国实验动物学报2012，Vol.20Issue(3)：56-59,后插6,5.DOI:10.3969/j.issn.1005-4847.2012.03.012
3,5-二氯苯基双胍抑制黑色素瘤肺转移的裸鼠体内药效学研究
Suppressive effect of 3, 5-dichlorophenylbiguanide on pulmonary metastasis of melanoma in nude mice
摘要
Abstract
Objective To observe the suppressive effect of 3, 5-dichlorophenylbiguanide on pulmonary metastasis of melanoma in vivo. Methods Human melanoma cell line M21 cells were injected into the tail vein of nude mice ( defined as day 1). The treatment procedure was carried out at days 5,9, 13, 17, and 21 by tail vein injection of 3,5-dichlorophenylbiguanide at a dose of 6 mg/kg, 12 mg/kg or 24mg/kg to the nude mice of the experimental groups. In addition, positive control, negative control, and vehicle control groups were set up. The mice were sacrificed at day 50. The lungs were removed and fixed in Bouin' s fixative solution for 24 h. Metastasis scores were figured out by a formula according to the diameters and numbers of white metastatic nodules on the lung surface. Results The 3,5-dichlorophenylbiguanide treatment reduced the metastasis scores to 55.25 ±13.60 (6 mg/kg), 35. 13 ±17.36 (12 mg/kg) and 12.83 ±11.44 (24 mg/kg) , significantly lower than that of the vehicle control group (P < 0.05). The cRGDfV treatment reduced metastasis score to 11. 50 ± 10.44, significantly lower than that of the vehicle control group (P < 0. 05). The metastasis score of the negative control IH1066 treatment group was 88. 50 ± 17. 21, and that of the untreated group was 88. 88 ± 11. 29, both with a non-significant difference compared with that of the vehicle control group (82. 50 ± 17. 72) (P >0.05). Conclusion 3,5-dichlorophenylbiguanide shows a suppressive effect on the pulmonary metastasis of melanoma in mice in a dose-dependent manner.关键词
3,5-二氯苯基双胍/黑色素瘤/转移/整合素αvβ3Key words
3, 5-dichlorophenylbiguanide/ Melanoma/ Metastasis/ Integrin ανβ33/ Nude mice分类
生物科学引用本文复制引用
张永慈,纪庆,杨铭,范冬梅,熊冬生,杨纯正,周圆..3,5-二氯苯基双胍抑制黑色素瘤肺转移的裸鼠体内药效学研究[J].中国实验动物学报,2012,20(3):56-59,后插6,5.基金项目
天津医科大学科学基金(2009ky05)和天津市应用基础研究重点项目(07JCZDJC04900)资助. （2009ky05）
