中国兽医科学2012，Vol.42Issue(7)：672-676,5.
捻转血矛线虫伊维菌素耐药株P-糖蛋白基因多态性的PCR-RFLP分析
Polymorphism analysis of P-glycoprotein gene in Haemonchus contortus of ivermectin resistance strains by PCR-RFLP
摘要
Abstract
To study the differences of P-glycoprotein gene between ivermectin-resistant（HY） and sus- ceptible strains（HM） of Haemonchus contortus and further clarify the relationship between P-glycoprotein gene and ivermectin resistance in future, the polymorphism of P-glycoprotein gene from ivermectin-resis- rant strain of H. contortus in sheep was analyzed by PCR-restriction fragment length polymorphism. The result showed that the PCR products of HM group were resistive to the restriction enzyme Kpn I , whileHY group were vulnerable to the enzyme. The statistics data showed that 10 nematodes of ivermectin-sus- ceptible strains were all homozygote（BB）;in 20 nematodes of ivermectin-resistance strain, there were 6 ivermectin-resistant homozygotes （AA, 30 %）, 13 heterozygotes （AB, 65% ） and only 1 ivermectin-suscepti- ble homozygote（BB, 5 %）. The heterozygote（AB） was the most dominant genotype, followed by ivermectin- resistant homozygotes（AA）,and ivermectin-susceptible homozygote（BB） was the least. The resistance al- lele A was the most dominant gene,and took 62.5%,while allele B was only 37.5% of the samples. The homologous analysis revealed that the P-glycoprotein gene of ivermectin resistance（hcpgp-1） shared low homology（80. 2% to 89. 3%） with the susceptible strains. Besides, mutant sites on the hcpgp-1 gene of ivermectin-resistant strains were also found. This paper could provide a reference for studying the effects of P-glycoprotein gene in ivermectin resistance. And it also could supply basic information for the mechanism study of resistance to common anthelmintics of H. contortus.关键词
捻转血矛线虫/耐药性/伊维菌素/P-糖蛋白/PCR-RFLPKey words
Haemonchus contortus/drug resistance/ivermectin/P-glycoprotein/PCR-RFLP分类
农业科技引用本文复制引用
刘鑫远,杨晓野,王瑞,杨莲茹,罗晓平,阿拉腾宝力格,贺志雄..捻转血矛线虫伊维菌素耐药株P-糖蛋白基因多态性的PCR-RFLP分析[J].中国兽医科学,2012,42(7):672-676,5.基金项目
内蒙古自然科学基金资助项目 （）
内蒙古农业大学博士基金资助项目 （）
内蒙古自治区高等学校科学研究项目 （）
