中国兽医科学2012，Vol.42Issue(7)：690-695,6.
口蹄疫病毒3A蛋白和FLAG标签的融合表达与血清学分析
Prokaryotic expression and serological analysis of FLAG-fused 3A protein of foot-and-mouth disease virus
摘要
Abstract
The FLAG tag,which consisted of 8 amino acids,was selected to make up the 10 amino acids deletion corresponding to residues 93 to 102 within 3A protein of foot-and-mouth disease virus（FMDV） vaccine strain OZK, and then the recombinant FLAG-fused 3A protein（3AF protein） was expressed in Escherichia coll. Meanwhile,the native 3A protein of wild type strain was also expressed as a control. Six to 8 week-old female BALB/c mice were vaccinated with 3A and 3AF as immunogens, the in vivo and in vitro serological reactivity of the proteins 3A and 3AF was also analyzed by ELISA and Western-blot,re- spectively. The results showed that both 3A and 3AF proteins were expressed effectively,primarily in solu- ble form. The anti-FLAG monoclonal antibody could specifically recognize the FLAG tag within the 3AF protein. Interestingly,both 3A and 3AF proteins could induce antibodies against 3A protein without anti- bodies against FLAG tag in mice,indicating the difference between the in vivo and in vitro reaction. The FALG tag made non significant difference in the expression level and expression form of 3A protein. The present study laid foundation for the rescue and application of FLAG-markered FMDV.关键词
口蹄疫病毒/3A蛋白/FLAG标签/酶联免疫吸附试验Key words
foot-and-mouth disease virus/3A protein/FLAG tag/ELISA分类
农业科技引用本文复制引用
祁国财,曹轶梅,付元芳,厍大亮,况文东,孙普,卢曾军,刘在新..口蹄疫病毒3A蛋白和FLAG标签的融合表达与血清学分析[J].中国兽医科学,2012,42(7):690-695,6.基金项目
国家高技术研究发展计划（863）项目 （）
