中国兽医科学2012，Vol.42Issue(7)：701-707,7.
Ⅰ群禽腺病毒抗体间接hexon—ELISA检测方法的建立
Establishment of an indirect hexon-ELISA for the detection of antibodies against fowl adenovirus group Ⅰ
摘要
Abstract
An indirect enzyme-linked immunosorbent assay（ELISA） method was developed to detect antibody against fowl adenovirus group Ⅰ （FAV Ⅰ ）. The recombinant protein hexon of FAV Ⅰ was used as coating antigen for the ELISA method,and the reaction conditions were optimized. The optimal concentra- tion of antigen was 10.0 μg/mL and the coating condition was 37 ℃ 2 h and 4℃ overnight. The optimal blocking buffer was 50 g/L skimmed milk,the optimal dilution and reaction time of serum was 1 ： 100 and 75 min,respectively;and the optimal dilution and reaction time of enzyme-labeled antibody were 1：2 000 and 45 min, respectively. The critical value of the hexon-ELISA was 0. 379. Furthermore, one hundred chicken serum samples were detected by the hexon-ELISA, and the positive rate of FAV I antibody was found to be 45%. The results suggested that the hexon-ELISA is a specific, sensitive and reliable method for the diagnosis, antibody level surveillance and epidemiologic investigation of FAV Ⅰ.关键词
Ⅰ群禽腺病毒/六邻体/酶联免疫吸附试验Key words
fowl adenovirus groupⅠ/hexon/ELISA分类
农业科技引用本文复制引用
罗思思,谢芝勋,刘加波,庞耀珊,邓显文,谢志勤,谢丽基,范晴,彭宜..Ⅰ群禽腺病毒抗体间接hexon—ELISA检测方法的建立[J].中国兽医科学,2012,42(7):701-707,7.基金项目
广西特聘专家专项经费资助项目 （）
广西科技项目 （）
