中国兽医科学2012，Vol.42Issue(7)：731-736,6.
合作猪TLR3及其剪接体的基因克隆与序列分析
Cloning and sequence analysis of TLR3 and its spliceosomes in Hezuo pig
摘要
Abstract
The objective of the present study was to investigate the alternative splicing of porcine Toll- like receptor 3（pTLR3） gene in Hezuo pig. The pTLR3 gene and spliceosomes（pTLR3a and pTLR3b） were cloned from porcine peripheral blood mononuclear cells by reverse transcription-polymerase chain re- action. Sequence analysis showed that the pTLR3 open reading frame was 2 718 bp in length encoded a de- duced protein with 905 amino acid residues. The sequence analysis of deduced amino acids indicated that it was a typical type I transmembrane protein with a LRRs ectodomain and a TIR cytoplasmic domain, the conserved structural features of TLRs. Due to the absence of extron 3, the spliceosomes of pTLR3a and pTLR3b could not encode the transmembrane and cytoplasmic domains. The similarity of nucleotide se- quence of pTLR3 with those of cattle, equine, cat and human was much higher than that of rabbit and mice. It was concluded that the alternative spliceosomes of pTLR3 do exist,and which lay foundations for further studies on their molecular functions.关键词
猪TLR3/克隆/序列分析/可变剪接Key words
porcine TLR3/cloning/sequence analysis/alternative splicing分类
生物科学引用本文复制引用
管齐赛,房永祥,贾怀杰,何小兵,周涛,景志忠..合作猪TLR3及其剪接体的基因克隆与序列分析[J].中国兽医科学,2012,42(7):731-736,6.基金项目
国家自然科学基金项目 （）
国家高技术研究发展计划（863）项目 （）
