中国药房2012，Vol.23Issue(24)：2284-2286,3.DOI:10.6039/j.issn.1001-0408.2012.24.31
HPLC法同时测定热毒清颗粒中绿原酸和连翘酯苷A的含量
Simultaneous Determination of Chlorogenic Acid and Forsythoside A in Reduqing Granules by HPLC
摘要
Abstract
OBJECTIVE: To establish a method for the simultaneous determination of chlorogenic acid and forsythoside A in Reduqing granules. METHODS: HPLC method was adopted. A Phenomenex Cn column was adopted with mobile phase consisted of methanol (A)-0.1% phosphoric acid solution (B) in gradient elution. The detection wavelength was set at 330 run. The flow rate was 1.0 mL·min-1, the injection volume was 10 μL and column temperature was 25℃. RESULTS: The linear ranges of chlorogenic acid and forsythoside A were 0.056 2~0.562 0 ug (r=0.999 9) and 0.041 l~0.411 0 ug (r=0.999 9), respectively. The average recoveries were 100.64% (RSD=0.51%) and 101.81% (RSD=0.74%), respectively. CONCLUSION: The method is simple and rapid, and can be used for the quality control of Reduqing granules.关键词
热毒清颗粒/绿原酸/连翘酯苷A/含量测定/高效液相色谱法Key words
Reduqing granules/ Chlorogenic acid/ Forsythoside A/ Content determination/ HPLC分类
医药卫生引用本文复制引用
鲍涵,张忠义,张军,黄宝明..HPLC法同时测定热毒清颗粒中绿原酸和连翘酯苷A的含量[J].中国药房,2012,23(24):2284-2286,3.基金项目
广东省教育部产学研结合项目(2010B090400135) （2010B090400135）
