中国药理学通报2012，Vol.28Issue(9)：1215-1217,3.DOI:10.3969/j.issn.1001-1978.2012.09.008
超氧阴离子对牛主动脉平滑肌细胞收缩性的影响
Effect of superoxide anion on contractility of aortic smooth muscle cells
摘要
Abstract
Aim To investigate the changes of [ Ca2 + ]; and contractility of aortic smooth muscle cells induced by superoxide anion. Methods The aortic smooth muscle cells were primarily cultured. The effect of superoxide anion on [ Ca2+ ]; was measured by a digital fluorescence microscopy system. Muscle contractility was analyzed by collagen gel contraction system. Results High-K+ and A-23187-induced [ Ca2+ ]; elevated both in control and X/XO-treated cells. The net increment of [ Ca2 + ]; was not significantly different between control and X/XO-treated cells( P >0. 05 ). Col- lagen gel contraction area in X/XO-treated cells induced by High-K+ and A-23187 was impaired from 18. 32% ±2. 11% to 5. 13% ±0. 45%( P <0. 01 )and 17. 16% ± 1. 10% to 4. 31% ± 0. 04% ( P < 0. 01 ). Conclusion The results demonstrate that superoxide anion attenuates smooth muscle contraction, whereas [ Ca2+ ]; increased by these agents were not affected.关键词
动脉平滑肌细胞/收缩性/细胞内钙/超氧阴离子/钙释放激活钙通道/电压依赖性钙通道Key words
aortic smooth muscle cell/ contractility/ intracellular calcium/ superoxide anion/ calcium release-activated calcium currents/ voltage dependent calcium channel分类
医药卫生引用本文复制引用
承伟,小山澈野,大池正宏..超氧阴离子对牛主动脉平滑肌细胞收缩性的影响[J].中国药理学通报,2012,28(9):1215-1217,3.基金项目
国家自然科学基金与国家留学基金委资助课题(No 30470765) （No 30470765）
