中国药理学通报2012，Vol.28Issue(9)：1229-1234,6.DOI:10.3969/j.issn.1001-1978.2012.09.011
木犀草苷对阿霉素诱导乳鼠心肌细胞损伤的保护作用
Protective effects of luteolin-7-0-glucoside on neonatal rat cardiomyocytes damage induced by adriamycin
摘要
Abstract
Aim To observe the protective effects of luteolin-7-0-g lucoside on neonatal rat cardiomyocytes induced by adriamycin ( ADR ) and explore its mechanism of the action. Methods Primary cardiomyocytes were isolated from neonatal rats and cultured for 48 h, the cells then were randomly divided into normal control groups, ADR model groups, ADR + luteolin-7-0-glucoside ( 12. 5, 6. 25, 3. 125 mg · L-1 ) simultaneously treated groups and ADR + luteolin-7-0-glucoside (12.5, 6.25, 3. 125 mg · L-1 ) pretreated for 4 h groups. The dose and time dependent effects of luteolin-7-0-glucoside on myocardial injury induced by ADR were investigated. The survival rate of myocardial cells was calculated by MTT assay. The leakage of lactate dehydrogenase ( LDH ), the contents of malondialde-hyde ( MDA ) and the activities of superoxide dimutase ( SOD ) were measured by respective kits. Results Compared with model groups, the survival rates of all luteolin-7-0-glucoside treated groups were significantly increased( P < 0. 05 ). The content of LDH leakage was reduced, the activities of SOD were increased, and the content of MDA was reduced in ADR + luteolin-7-0-glucoside ( 12. 5, 6. 25 mg · L-1 ) groups( P <0. 05 ). In the luteolin-7-0-glucoside 4 h pre-incubation groups the contents of LDH leakage were significantly reduced , activities of SOD were increased, and the contents of MDA were reduced( P < 0. 01 ). Conclusion Luteolin-7-0-glucoside shows significant protective effects on myocardial damage induced by ADR, and the protective effects can be improved after 4 h s' pre-treatment with luteolin-7-0-glucoside. The mechanism of this may be related to the effects of anti-free radical and the inhibition of lipid peroxidation.关键词
木犀草苷/心肌细胞/阿霉素/抗氧化/LDH/MDA/SODKey words
luteolin-7-0-glucoside/ cardiomyocytes/ adriamycin / antioxidant / LDH/ MDA/ SOD分类
医药卫生引用本文复制引用
何玲,孙桂波,孙潇,孙静,孙晓波..木犀草苷对阿霉素诱导乳鼠心肌细胞损伤的保护作用[J].中国药理学通报,2012,28(9):1229-1234,6.基金项目
国家科技支撑计划项目(No 2008BAI51B02) （No 2008BAI51B02）
国家重大新药创制科技专项(No 2009ZX09502～015)国家科技部科研院所技术开发研究专项(No 2008EG150298) （No 2009ZX09502～015）
国家重点基础研究发展计划(973计划)项目(No 2009CB522805) （973计划）
