中国药理学通报2012，Vol.28Issue(9)：1234-1238,5.DOI:10.3969/j.issn.1001-1978.2012.09.012
小檗碱抑制HCT116细胞生长与Wnt/β-catenin信号的关系研究
Study on the correlation between the anti-proliferation effect of berberine on HCT116 cells and Wnt/β-catenin signaling
摘要
Abstract
Aim To investigate the correlation between the anti-proliferation effect of berberine on HCT116 cells and Wnt/β-catenin signaling transduction. Methods Crystal violet staining was employed to detect the anti-proliferation effect of berberine on HCT116 cells. The apoptosis induced by berberine was detected by fluorescence activated cell sorting and Western blot. The p-catenin expression was measured by Western blot and reverse transcription and polymerase chain reaction ( RT-PCT ). With exogenous expression of β-catenin,the role of p-catenin in berberine induced proliferation inhibitory effect on HCT116 cells was explored. Results Berberine could inhibit the prolifera- tion and induce apoptosis in HCT116 cells. The expression of β-catenin protein in whole cell, cytoplasm and nucleus was inhibited when treated HCT116 cells with different concentrations of berberine. So was the mRNA expression level of β-catenin. Exogenous expression of β-catenin could attenuate the anti-proliferation effect of berberine on HCT116 cells. Conclusions Berberine can inhibit the proliferation of HCT116 cells and Wnt/β-catenin signaling transduction. This effect may be mediated at least by down regulating the mRNA expression of β-catenin.关键词
小檗碱/HCT116细胞/增殖抑制/凋亡/Wnt/β-catenin/信号通路Key words
berberine/ HCT116 cell/ proliferation inhibition/ apoptosis/ Wnt/p-catenin/signaling pathway分类
医药卫生引用本文复制引用
杨秋珺,周龙洋,刘映孜,牟钰钦,吴柯,周岐新,罗进勇,何百成..小檗碱抑制HCT116细胞生长与Wnt/β-catenin信号的关系研究[J].中国药理学通报,2012,28(9):1234-1238,5.基金项目
国家自然科学基金资助项目(No 81071462) （No 81071462）
重庆市自然科学基金资助项目(No 2011BB5129) （No 2011BB5129）
