中国药理学通报2012，Vol.28Issue(9)：1262-1266,5.DOI:10.3969/j.issn.1001-1978.2012.09.018
肉桂酸对人骨肉瘤MG-63细胞增殖和分化的影响
Effect of cinnamic acid on proliferation and differentiation of human osteosarcoma MG-63 cells
摘要
Abstract
Aim To investigate the effect of cinnamic acid on proliferation and differentiation of human osteosarcoma MG-63 cells. Methods MG-63 cells were treated with cinnamic acid. The living cells were counted by trypan blue staining. The morphology and ultra-structure of MG-63 cells were studied with optical microscope and electron microscope. The changes of cell cycle were analyzed by flow cytometry ( FCM ). The expression of type I collagen was detected by Van Gie-son staining. The formation of mineralized bone nodules was evaluated by alizarin red staining. The expressions of osteocalcin and osteonectin were detected by immunocytochemical staining. Results Cinnamic acid inhibited the proliferation of MG-63 cells significantly, the inhibitory rate was 54. 94% ± 4. 69% after treatment with cinnamic acid for 7 days. Flow cytometry a- nalysis showed that cinnamic acid increased the cell numbers in G0/G1 phase significantly( P <0. 01 ). Under optical microscope and electron microscope, the morphology and ultrastructure of MG-63 cells treated with cinnamic acid changed significantly compared with those in control group, showing the characteristics of normal cells. Furthermore, cinnamic acid promoted the expressions of osteoblast markers such as type I collagen, osteonectin and osteocalcin, increased calcium aggradation and facilitated the formation of bone nodules. Conclusion Cinnamic acid can inhibit the proliferation of human osteosarcoma MG-63 cells and induce the differentiation of MG-63 cells into osteoblasts.关键词
肉桂酸/人骨肉瘤MG-63细胞/增殖/分化/骨肉瘤/成骨细胞Key words
cinnamic acid/ human osteosarcoma MG-63 cells/ proliferation/ differentiation/ osteosarcoma/ osteoblasts分类
医药卫生引用本文复制引用
王国红,郭直岳,石松林,李祺福..肉桂酸对人骨肉瘤MG-63细胞增殖和分化的影响[J].中国药理学通报,2012,28(9):1262-1266,5.基金项目
国家自然科学基金资助项目(No 81071669) （No 81071669）
