中国药理学通报2012，Vol.28Issue(9)：1267-1272,6.DOI:10.3969/j.issn.1001-1978.2012.09.019
溴化乙锭对布鲁姆综合症解旋酶生物学特性的影响
Effects of ethidium bromide on the biological properties of Bloom syndrome helicase
摘要
Abstract
Aim To study the effects of ethidium bro-mide( EB ) on biological properties of BLM helicase. Methods Effects of EB on DNA-binding activity and helicase activity of BLM helicase were detected by fluorescence polarization; effect on ATPase activity was detected by free phosphorus assay technology; effect on conformation was detected by ultraviolet Absorption spectrum. Results DNA-binding activity of BLM helicase was completely inhibited by EB. Ci was respectively ( 21. 3 ± 0. 7 ) μmol · L-1 and ( 3. 3 ± 0. 3 ) μmol · L-1 when dsDNA and ssDNA reacted as the substrates; helicase activity was also completely inhibited and Ci was ( 9. 0 ± 0. 3 ) μmol · L-1; ATPase activity was inhibited mildly and the disparity was not obvious ; conformation of the helicase was changed, and the maximum UV absorption had been shifted. Conclusion EB bound to BLM helicase may change the conformation, inhibit its binding to DNA, and further inhibit the biological activities of the helicase.关键词
溴化乙锭/BLM解旋酶/DNA结合活性/解链活性/ATPase活性/构象Key words
ethidium bromide/ BLM helicase/ DNA binding activity/ helicase activity/ ATPase activity/ conformation分类
医药卫生引用本文复制引用
许庆贺,许厚强,骆衡,陈祥,张金彪,李坤..溴化乙锭对布鲁姆综合症解旋酶生物学特性的影响[J].中国药理学通报,2012,28(9):1267-1272,6.基金项目
国家重点基础研究发展计划(973计划)资助项目(No 2010CB534912) （973计划）
教育部博士点基金资助项目(No 200806570003) （No 200806570003）
贵州省国际科技合作计划项目(黔科合外G字[2011]7008号) （黔科合外G字[2011]7008号）
贵州省优秀人才省长资金资助项目(No 200822) （No 200822）
贵州大学SRT项目(No 2010024) （No 2010024）
