中国药理学通报2012，Vol.28Issue(9)：1281-1285,5.DOI:10.3969/j.issn.1001-1978.2012.09.022
毒胡萝卜素对人胚肺成纤维细胞凋亡影响及部分机制的初步探讨
Effect and mechanism of thapsigargin in apoptosis in MRC-5 cells
摘要
Abstract
Aim To study changes of endoplasmic re-tieulum stress relevant protein on the apoptosis in human fetal lung fibroblast cells ( MRC-5 ) and explore the apoptotic effect of thapsigargin ( TG ) on MRC-5 through ER stress activation. Methods Apoptotic rate in MRC-5 cells was observed using flow cytometric a-nalysis. The expression levels of GRP78, CHOP mR-NA was evaluated by RT-PCR. The protein levels of GRP, CHOP, Caspase-3 were assessed by Western blot. Results The apoptotic rates of MRC-5 cells were significantly increased, and were statistically signifi- cant when compared with the control ( P < 0. 05 ). GRP, CHOP mRNA and proteins levels were significantly increased when the cells were separately treated with TG for 24 h when compared with the control ( P < 0. 05 , P < 0. 01 ). Meanwhile, the protein expression of Caspase-3 in TG was also upregulated. Conclusions The elevated expression of relevant proteins when ERS occurs indicates ERS participates in the process of apoptosis in MRC-5 cells.关键词
内质网应激/人胚肺成纤维细胞/毒胡萝卜素/GRP/CHOP/Caspase-3Key words
endoplasmic reticulum stress/ MRC-5 cell/ TG/ GRP/ CHOP/ Caspase-3分类
医药卫生引用本文复制引用
王亚丽,李俊,黄成,黄艳,于明哲,任丹阳,徐涛..毒胡萝卜素对人胚肺成纤维细胞凋亡影响及部分机制的初步探讨[J].中国药理学通报,2012,28(9):1281-1285,5.基金项目
国家自然科学基金资助项目(No 81102493,30973917) （No 81102493,30973917）
安徽省自然科学基金资助项目(No 090413134) （No 090413134）
